Biological microemulsion micelle, bladder cancer cell suspension and mouse air sac bladder cancer model construction method

Abstract

The invention belongs to the technical field of cancer model construction and discloses biological microemulsion micelle, bladder cancer cell suspension and a mouse air sac bladder cancer model construction method. The biological microemulsion micelle is prepared by steps: preparing PAMAM into 10% PAMAM solution by tumor growth promoting liquid; adding COOH-PEG-OH into the 10% PAMAM solution, and well mixing; mixing 5% aldehyde dextran with the above solution to obtain the biological microemulsion micelle. The cell suspension is obtained by mixing of digested log-phase bladder cancer cells and the biological microemulsion micelle. The bladder cancer cell suspension enables tumor clustering growth, prevents cancer cell migration and promotes tumor growth. The air sac bladder cancer model is characterized in that an air sac is adopted for replacement of a bladder and can be used for medicine perfusion, and high success rate and short tumor forming period are realized.

Description

technical field [0001] The invention relates to a method for constructing biological microemulsion micelles, bladder cancer cell suspension and mouse airbag bladder cancer model, and belongs to the technical field of cancer model construction. Background technique [0002] In the establishment of existing mouse tumor models, biological glue (matrigel, matrigel) is often used to prepare cell suspension to facilitate cell aggregation and increase the success rate of tumor planting. However, when using biological glue, tumor cells sometimes aggregate in the suspension and cannot Getting enough nutrition will eventually cause the tumor to fail. At the same time, bioglue will cause ischemia and hypoxia of tumor mass, resulting in slow or even stagnation of tumor growth. At present, mouse bladder cancer models are divided into subcutaneous tumor models and orthotopic tumor models. Subcutaneous tumor models are mostly completed by planting tumor cells subcutaneously. Orthotopic t...

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problem that the existing biological glue is used to prepare the cell suspension for seeding the tumor, the tumor cells are aggregated in the suspension and cannot obtain enough nutrition, which eventually leads to the failure of the seeding tumor, or the lack of nutrient supply caused by the tumor mass, and in the mouse bladder cancer model. The subcutaneous tumor model cannot use the way of drug infusion, and the side effects and curative effects of drug infusion cannot be accurately evaluated. The mortality rate of the transplanted tumor model is high, and the modeling period of the spontaneous tumor model is too long. The present invention provides a method for constructing a bladder cancer model The cell suspension, the preparation method of the air pouch, the construction method of the air pouch bladder cancer model and the construction method of the mouse pouch bladder cancer model, the air pouch bladder cancer model can be infused with drugs, which can accurately evaluate the curative effect and side effects of the drug, and the model The tumor formation rate is high, the mortality rate is low, and the model construction time is short. The technical scheme adopted is as follows:

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