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A strain of Pseudomonas arcticus with antifungal activity

A technology of alternating Pseudomonas and fermenting bacteria, applied in the field of microbial screening, can solve problems such as loss

Active Publication Date: 2020-07-10
THE FIRST INST OF OCEANOGRAPHY SOA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Especially in the case of low temperature, high humidity and poor ventilation conditions in the winter warm shed, once the disease occurs, if it cannot be prevented and controlled in time, it will cause serious losses.

Method used

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  • A strain of Pseudomonas arcticus with antifungal activity
  • A strain of Pseudomonas arcticus with antifungal activity
  • A strain of Pseudomonas arcticus with antifungal activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1. Screening of bacterial strains

[0021] Pseudoalteromonas sp.BJ01 was derived from sea mud samples collected during China's 6th Arctic Scientific Expedition. Zobel 2216E medium was used for streak screening, purification, and 16S rDNA species identification. The purified strains were respectively inserted into Erlenmeyer flasks equipped with 2216E liquid medium, and cultured with shaking at 25°C and 150rpm for 24h to be used as bacterial suspension.

[0022] For the screening of antibacterial strains, common plant pathogenic bacteria such as Fusarium oxysporum were selected as indicator bacteria, plant pathogenic fungi were inoculated on PDA solid medium, and after culturing at 25°C for 48 hours, a piece of mycelium was taken and added to the sterile Shake fully in the test tube of water, after filtering mycelia with two layers of sterilized gauze, dilute to obtain phytopathogenic fungal spore suspension (10 5 cell / mL). The antibacterial activity of the str...

Embodiment 2

[0027] Example 2. Biochemical characteristics of Pseudoalteromonas sp.BJ01 strain

[0028] After multiple times of streak separation, the purified BJ01 (see figure 1 ), identified as Pseudoalteromonas sp. The bacteria are Gram-negative, white, round, moist colonies. Then the representative strains were tested for physiology and biochemistry using API 20 NE identification strips, the culture temperature was set at 10°C, and the time for reading the results was 48 hours. The results showed that the bacteria could utilize a variety of substrates (see Table 2).

[0029] Table 2 Physiological and biochemical assay results of Arctic strain BJ01API

[0030] NO3 TRP GL U

Embodiment 3

[0031] Embodiment 3. The biocontrol effect of Pseudoalteromonas sp.BJ01 bacterial strain at different temperatures

[0032] The fermentation liquid of Pseudoalteromonas sp.BJ01 strain is properly added with nutrients necessary for plant growth to make a fungicide, and stored at 4°C for future use. The bactericidal preparation is a light yellow liquid, and the number of viable bacteria reaches 10 9 cell / ml.

[0033] Pseudoalteromonas sp.BJ01 fermentation broth (diluted 1:1) was sprayed with a sprayer, and the liquid was evenly sprayed on the whole plant of the infected cucumber, and the sprayed cucumbers were placed at 10°C, 20°C and 30°C respectively In the light incubator at ℃, the test sprayed 2 times with an interval of 7 days. "Milzhi" 50ml + "Garlic Oil" 15-20ml mixed with 15kg of water was diluted as the positive control, and water was used as the blank control, and each treatment was repeated three times.

[0034] Table 3 Control effect of Pseudoalteromonas sp.BJ01 f...

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Abstract

The invention provides a strain of Pseudomonas arcticus with antifungal activity, and its preservation number is CGMCC No.14505. The application of the screened pseudomonas in the preparation of phytopathogenic inhibitor products. BJ01, Pseudoalteromonas sp.BJ01 provided by the present invention, its antibacterial experiment shows that the bactericidal preparation has inhibitory effect on various plant pathogens, and the control effect on greenhouse cucumber gray mold is as high as 66.7% under low temperature conditions of 10-15°C %. The bacterial strain of the invention has potential application prospects in the pollution-free biological control of plant diseases.

Description

technical field [0001] The invention belongs to the technical field of microbial screening, and in particular relates to a strain of arctic pseudomonas with antifungal activity. Background technique [0002] Crop diseases caused by fungi have become an important issue threatening global food security. The increase in the variety and quantity of crops, the global trade and transportation of crops, the changes in global climate and environment, and the adaptation of pathogenic fungi to new ecosystems have all led to the increasing severity of fungal diseases. At present, artificially synthesized fungicides are still the main and most effective means to prevent and control fungal diseases of crops, but they also cause harm to humans and the environment. Microbial control of crop fungal diseases has the advantages of safety, high efficiency, and environmental friendliness, and has become an alternative to chemical fungicides that has attracted widespread attention at home and a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/20A01P3/00C12R1/01
CPCA01N63/00C12N1/205C12R2001/01
Inventor 李江何培青
Owner THE FIRST INST OF OCEANOGRAPHY SOA
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