A method for continuous submerged fermentation to extract active substances from edible fungi

A technology of submerged fermentation and active substances, applied in the field of fermentation engineering, can solve the problems of low utilization rate of active ingredients, shorten the cultivation period, reduce the production period, etc., and achieve the effect of accelerating the growth rate of bacteria, shortening the cultivation period and reducing the production period

Active Publication Date: 2018-07-31
济宁利马菌业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention aims at the problems of complex process and low utilization rate of active ingredients in extracting active substances of edible fungi by submerged fermentation at present, and provides a method for extracting active substances of edible fungi by continuous submerged fermentation. High-voltage pulsed electric field assists submerged fermentation, which is easy to operate. It can not only shorten the cultivation period, obtain high-yield and strong stress-resistant bacteria, but also fully extract the active ingredients of edible fungi, reduce the number of cell death during the fermentation process, and increase the number of production. , realize continuous operation, reduce production cycle, and have great development prospects

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] F1: In this example, 50 g of white fungus fruiting bodies were taken, washed, dried, and crushed to 100 mesh to obtain white fungus powder, mixed with water and uniformly sterilized according to the ratio of material to liquid at 1:50, and the seed material was obtained.

[0041] F2: The seed liquid of this example is inoculated into the sterilized compound amino acid medium according to the inoculum amount of 15% by weight, and then placed on a shaker and passed through an electromagnetic field for cultivation. The cultivation temperature is 25°C. After 36 hours, the seeds are obtained. liquid;

[0042] In this embodiment, the electromagnetic field intensity is 15mT, and the action time is 15min / h;

[0043] In this embodiment, the seed liquid culture medium is based on 1000ml of water, 15 parts of soluble starch, 30 parts of glucose, KH 2 PO 4 1 part MgSO 4 •7H 2 O 0.8 parts, glutamic acid and glycine 1:1 combination 0.6 parts; adjust the pH to 6.0, stir well, add ...

Embodiment 2

[0056] F1: In this example, 50 g of ganoderma lucidum fruiting bodies were taken, washed, dried, and crushed to 100 mesh to obtain ganoderma lucidum powder, and the ganoderma lucidum powder was mixed with water at a ratio of 1:50 to uniformly sterilize the ganoderma lucidum seed material.

[0057] F2: The seed liquid of this example is inoculated into the sterilized compound amino acid medium according to the inoculum amount of 15% by weight, and then placed on a shaker and passed through an electromagnetic field for cultivation. The cultivation temperature is 28°C. After 72 hours, the seeds are obtained. liquid;

[0058] In this embodiment, the electromagnetic field intensity is 20mT, and the action time is 20min / h;

[0059] In this embodiment, the seed liquid culture medium is based on 1000ml of water, 20 parts of soluble starch, 40 parts of glucose, VB 2 0.2 copies, VB 6 0.1 parts, KH 2 PO 4 1 part MgSO 4 •7H 2 O 1 part, 0.5 parts of glutamic acid and glycine 1:1 comb...

Embodiment 3

[0072] F1: 10cm of Cordyceps sinensis mycelium was taken in this embodiment 2 , and water according to the material-to-liquid ratio of 1:50, you can get the seed material.

[0073] F2: The seed solution of this example is inoculated into the sterilized compound amino acid medium according to the inoculum amount of 10% by weight, and then placed on a shaker and fed into an electromagnetic field for cultivation. The cultivation temperature is 22°C. After 72 hours, the seeds are obtained. liquid;

[0074] In this embodiment, the electromagnetic field intensity is 20mT, and the action time is 10min / h;

[0075] In this embodiment, the seed liquid culture medium is based on 1000ml of water, 20 parts of soluble starch, 40 parts of glucose, 0.8 part of yeast powder, 0.2 part of silkworm chrysalis powder, KH 2 PO 4 0.8 parts, MgSO 4 •7H 2 0.8 parts of O, 0.4 parts of glutamic acid and glycine 1:1 combination; adjust the pH to 5.5, stir well, add 0.6 parts of glycine and serine 1:1...

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Abstract

The invention provides a method for continuous submerged fermentation to extract active substances of edible fungi. The edible fungus mycelium or fruiting body is assisted by an external electromagnetic field to carry out seed liquid cultivation, and then the seed liquid bacteria are immobilized and made with Magnetic, the immobilized bacteria are subjected to submerged fermentation with the assistance of a high-voltage pulsed electric field. The edible fungus active substance obtained by the invention has the following advantages: the active ingredients of the edible fungus are fully extracted, and the production cycle is greatly reduced. The immobilized bacteria can be fermented multiple times, and the magnetically immobilized bacteria are beneficial to the separation and purification of products, reducing the burden on downstream technologies; improving the long cycle of extraction of edible fungus active substances, low utilization of active ingredients, and contamination problems such as excessive bacteria, and provide a technical basis for subsequent diversified production.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering, and in particular relates to a method for extracting active substances of edible fungi through fermentation technology. Background technique [0002] As a higher fungus with dual functions of food and medicine, edible fungus is one of the most potential microbial species. Edible fungus fruiting body cultivation has a long history, but artificial cultivation takes a long time, the technology is complex, and is limited by seasons and raw materials. Therefore, edible fungus liquid submerged fermentation technology came into being. There are two target products of submerged fermentation of edible fungi: one is liquid strains, which replace solid strains and put into production to obtain fruiting bodies; the other is mycelium and its metabolites, which obtain physiological activities that fruiting bodies cannot produce or whose content is higher than that of fruiting bodies substance...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N11/04C12N11/10C12N13/00C12P1/02C12R1/645
CPCC12N1/14C12N11/04C12N11/10C12N13/00C12P1/02C12N1/145C12R2001/645
Inventor 戚海冰
Owner 济宁利马菌业股份有限公司
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