Application of enzymatic hydrolysates of total glycosides of grosvenor momordica fruit to anti-hepatic fibrosis drugs
A technology of total glycoside enzymolysis product and liver fibrosis, which is applied in the field of medical use of Chinese medicinal material extract mogroside enzymolysis product for anti-liver fibrosis, can solve the problems such as no application report of total glycoside enzymolysis product of Mogroside, and achieves The effect of extracts with clear activity, low cost and wide practical value
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Embodiment 1
[0033] Example 1 Mogroside total glycosides hydrolyzate to CCl 4 Effects of induced liver fibrosis in mice
[0034] 1. Materials and Instruments
[0035] ICR mice, female, weighing 18-22 g (provided by Nanjing Qinglongshan Animal Breeding Farm). Wahaha purified water; α-SMA monoclonal antibody (abcam); goat anti-rabbit IgG (bioworld).
[0036] 2. Experimental method
[0037]ICR female mice were divided into blank group, model group, MG-L group (125mg / kg), MG-H group (250 mg / kg), MG-EH-L group (125mg / kg), MG-EH-H group group (250 mg / kg), the drugs were administered by intragastric administration, 20 rats in each group. Mice were injected intraperitoneally with 40% CCl4 (olive oil suspension) 2ml / kg, twice a week to establish a mouse model of liver fibrosis; the blank group was injected intraperitoneally with olive oil 2ml / kg, twice a week. At the same time as the model was established, the drug was given to the stomach for treatment. The mice were killed at the 6th week af...
Embodiment 2
[0046] Example 2 Effect of MG and MG-EH on the expression of α-SMA protein in HSC-T6 induced by TGF-β1
[0047] HSC-T6 is the effector cell of liver fibrosis. Liver fibrosis is the result of the joint action of various cytokines, and the key step is the continuous inflammation to activate HSC and cause a large amount of ECM deposition. Transforming growth factor-β (TGF-β) is a key factor in promoting liver fibrosis, which can activate HSC-T6, and the activated HSC-T6 can release cytokines through autocrine or paracrine, resulting in a large amount of ECM deposition, which eventually causes hepatic fibrosis. fibrosis. In this experiment, the degree of liver fibrosis was judged by detecting the content of α-SMA protein.
[0048] HSC-T6 was purchased from Nanjing Kaiji Biotechnology Development Co., Ltd. HSC-T6 was cultured in DMEM medium containing 10% domestic calf serum in a 37°C, 5% CO2 incubator. The medium was changed every 2 days, and the cells were digested with 0.25% ...
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