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Rapid propagation method of Thunia alba seedlings

A seedling and rapid technology, applied in the field of rapid propagation of bamboo shoots, can solve the problems that resources cannot meet market demand, it is difficult to achieve large-scale production, and the reproduction speed of bamboo shoots is slow. Low cost and uniform seedlings

Active Publication Date: 2017-12-15
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the wild resources of bamboo shoots are exhausted, and the existing resources can no longer meet the current market demand.
[0003] Existing bamboo shoot orchid seedling propagation technology mainly adopts division propagation method, this method is slow in propagation speed of bamboo shoot orchid seedling, seedling quality is uneven, operation method is complicated, cost is high, standardization degree is low, it is difficult to realize large-scale production

Method used

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  • Rapid propagation method of Thunia alba seedlings
  • Rapid propagation method of Thunia alba seedlings
  • Rapid propagation method of Thunia alba seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] A kind of method for rapid propagation of bamboo shoot orchid seedlings, the steps are as follows:

[0056] a. Select the stem tip section of wild bamboo shoot orchid to obtain product A; the shoot tip section of the wild bamboo shoot orchid is selected from the tender part of the stem tip of the wild bamboo shoot orchid plant that has no yellow leaves and no disease in May to June;

[0057] b. Rinse product A with clean water, rinse twice with sterile water, each time for 10s, soak in 75% medical alcohol for 15s, wash with sterile water, and then use HgCl 2 The solution was sterilized twice, each time for 5 minutes, and the HgCl was washed with sterile water 2 , to obtain product B; the HgCl 2 The solution concentration is 0.1%;

[0058] c. Culture medium preparation: preparation of value-added medium C 1 rooting medium C 2 product, the value-proliferating medium C 1 The ingredients in the product include: MS+30g / L sucrose+6g / L agar+0.3g / L activated carbon powder+...

Embodiment 2

[0062] A kind of method for rapid propagation of bamboo shoot orchid seedlings, the steps are as follows:

[0063] a. Select the stem tip section of wild bamboo shoot orchid to obtain product A; the shoot tip section of the wild bamboo shoot orchid is selected from the tender part of the stem tip of the wild bamboo shoot orchid plant that has no yellow leaves and no disease in May to June;

[0064] b. Rinse product A with clean water, rinse 3 times with sterile water, each time for 15s, soak in 75% medical alcohol for 20s, wash with sterile water, and then use HgCl 2 The solution was sterilized twice, each time for 8 minutes, and the HgCl was washed with sterile water 2 , to obtain product B; the HgCl 2 The solution concentration is 0.15%;

[0065] c. Culture medium preparation: preparation of value-added medium C 1 rooting medium C 2 product, the value-proliferating medium C 1 The ingredients in the product include: MS+30g / L sucrose+6g / L agar+0.3g / L activated carbon powd...

Embodiment 3

[0069] A kind of method for rapid propagation of bamboo shoot orchid seedlings, the steps are as follows:

[0070] a. Select the stem tip section of wild bamboo shoot orchid to obtain product A; the shoot tip section of the wild bamboo shoot orchid is selected from the tender part of the stem tip of the wild bamboo shoot orchid plant that has no yellow leaves and no disease in May to June;

[0071] b. Rinse product A with clean water, rinse twice with sterile water, each time for 15s, soak in 75% medical alcohol for 18s, wash with sterile water, and then use HgCl 2 The solution was sterilized twice, 6 minutes each time, and the HgCl was washed with sterile water 2 , to obtain product B; the HgCl 2 The solution concentration is 0.13%;

[0072] c. Culture medium preparation: preparation of value-added medium C 1 rooting medium C 2 product, the value-proliferating medium C 1 The ingredients in the product include: MS+30g / L sucrose+6g / L agar+0.3g / L activated carbon powder+60g...

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Abstract

The invention discloses a rapid propagation method of Thunia alba seedlings. The rapid propagation method comprises the following steps: (a) selecting stem tips of wild Thunia alba, so as to obtain a product A; (b) cleaning the product A with clean water, flushing the product A with sterile water, soaking into 75% medical alcohol, cleaning with sterile water, disinfecting the product A with an HgCl2 solution, and cleaning HgCl2 with sterile water, so as to obtain a product B; (c) preparing a culture medium, so as to obtain a proliferated a culture medium product C and a rooting culture medium product D; and (d) carrying out seedling formation culturing on Thunia alba, inoculating the product B into the product C1 for culture, and inoculating the product C1 to the product C2 for culture, so as to obtain the seedlings. According to the rapid propagation method, the rapid propagation of the Thunia alba seedlings can be realized, and the disease resistance, stability and regularity of the seedlings are guaranteed; the method is simple in operation, low in cost, high in standardization level and applicable to large-scale production.

Description

technical field [0001] The invention relates to a method for rapid propagation of plant seedlings, in particular to a rapid propagation method for bamboo shoot orchid seedlings. Background technique [0002] Sun orchid (Thunia alba (Lindl.) Rchb.f.), also known as Tonglan, belongs to the ground or epiphytic herbaceous plant of the genus Thunia in the family Orchidaceae. Sun orchid blooms in summer, with terminal racemes, large, beautiful and fragrant flowers. It is mainly distributed in the southwest to the south of Yunnan and the east of Tibet in my country, and grows on rocks in the forest or in the recesses of tree branches. At present, the demand for bamboo shoots orchids in the market is mainly manifested in two aspects: ornamental and medicinal. The flower culture of orchids in my country has a long history, and orchids have high ornamental value, and are known as one of the four gentlemen of flowers; Medicinal materials (Miao medicine), the whole plant is used as med...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 黄明进钱安桥罗春丽张明生赵致
Owner GUIZHOU UNIV
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