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A kind of breeding method of cattle vigorously

A breeding method, Niu Dali's technology, applied in horticultural methods, botanical equipment and methods, cultivation, etc., can solve the problems of Niu Dali's low survival rate, long time for slowing down seedlings, yellowing of leaves, etc., to avoid drought and dehydration No germination, well-developed root system, and good plant shape

Active Publication Date: 2019-12-31
台州中知英健机械自动化有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, with the rapid increase in market demand, the research on the breeding technology of cattle vigorously seedlings has gradually deepened and comprehensively, mainly including sowing seedlings, cutting seedlings and tissue cultivation seedlings. Tissue cultivation seedlings are one of the effective methods for large-scale reproduction of cattle vigorously. 1. At present, most young stems are used as explants, and it is difficult to sterilize. There have been studies on the rapid propagation of tissue culture by using seeds as materials through the callus method, but there is no such thing as using seeds as materials to propagate cattle vigorously through the method of bud propagation. Report on the study of tissue culture rapid propagation
In addition, artificially planted Niu Dali often has dead seedlings, yellow leaves, slow seedlings for a long time, and no growth. The overall survival rate of Niu Dali is not high.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A method for cultivating cattle vigorously, comprising the following steps: collecting and processing seeds, tissue culture, sowing seedlings, transplanting, water and fertilizer management and pruning between seedlings.

[0033] a. Seed collection and processing

[0034] After the fruit matures, choose a mother tree that is robust and free of diseases and insect pests. After collecting the fruit, peel off the husk, take out the seeds, soak them in 40°C water for 2 days, and then stir them with 2.5% Shileshi suspension agent for 10 minutes. Use 0.1% HgCl on the bench 2 The solution was disinfected for 8-10 minutes, then soaked in 75% ethanol for 10 seconds, and finally washed 4 times with sterile water, and set aside.

[0035] b. Tissue culture

[0036] b.1 Primary culture: inoculate the seeds treated in step a in the germination medium, germinate aseptically for 7 days, light for 12 hours every day, and the temperature is 22°C; the composition of the germination medi...

Embodiment 2

[0049] A method for cultivating cattle vigorously, comprising the following steps: collecting and processing seeds, tissue culture, sowing seedlings, transplanting, water and fertilizer management and pruning between seedlings.

[0050] a. Seed collection and processing

[0051] After the fruit matures, choose a mother tree that is robust and free of diseases and insect pests. After collecting the fruit, peel off the husk, take out the seeds, soak them in 50°C water for 4 days, and then stir them with 3.5% Shileshi suspension agent for 15 minutes. Use 0.2% HgCl on the bench 2 The solution was disinfected for 10 minutes, then soaked in 85% ethanol for 15 seconds, and finally rinsed with sterile water for 6 times, and set aside.

[0052] b. Tissue culture

[0053] b.1 Primary culture: Inoculate the seeds treated in step a in the germination medium, germinate aseptically for 9 days, light for 15 hours every day, and the temperature is 26°C; the composition of the germination me...

Embodiment 3

[0066] A method for cultivating cattle vigorously, comprising the following steps: collecting and processing seeds, tissue culture, sowing seedlings, transplanting, water and fertilizer management and pruning between seedlings.

[0067] a. Seed collection and processing

[0068] After the fruit matures, choose a mother tree that is robust and free from diseases and insect pests. After collecting the fruit, peel off the shell, take out the seeds, soak them in 50°C water for 3 days, and then stir them with 3% Shileshi suspension agent for 12 minutes. Use 0.15% HgCl on the bench 2 The solution was disinfected for 9 minutes, then soaked in 80% ethanol for 12 seconds, and finally rinsed with sterile water for 5 times, and set aside.

[0069] b. Tissue culture

[0070] b.1 Primary culture: Inoculate the seeds treated in step a in the germination medium, germinate aseptically for 8 days, light for 13 hours every day, and the temperature is 25°C; the composition of the germination m...

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Abstract

The invention belongs to the technical field of agricultural planting. A cultivation method of millettia speciosa champ comprises the following steps: a. collecting and treating seeds; b. carrying out tissue culture; c. sowing and breeding seedlings; d. transplanting; e. carrying out water and fertilizer management; f. pruning the seedlings. The cultivation method of the millettia speciosa champ takes the seeds as a material and realizes tissue culture speed propagation of the millettia speciosa champ in a way of bud propagation; the seedlings are obtained by the steps of primary culture, proliferative secondary culture, strong seedling cultivation and rooting culture; the obtained seedlings do not have the phenomenon of dehydration. The millettia speciosa champ cultivated by the method is good in growth and development, high in plant height and strong as well as developed in seedling root system, has thick and strong rattans, and is rapid in growth, more in tuberization, easy to manage and high in disease resistance.

Description

technical field [0001] The invention belongs to the technical field of agricultural planting, and in particular relates to a method for cultivating cattle vigorously. Background technique [0002] Niu Dali is a medicinal and edible plant of Papilionaceae Papilionaceae, also known as beautiful twig bean vine, sweet cow vigorously, grilled mountain tiger, vigorous potato, and mountain lotus root. Niu Dali uses its roots as medicine, and its main components are protein and starch quality and alkaloids etc. Niu Dali mainly treats lung heat, lung deficiency cough, tuberculosis, rheumatoid arthritis, lumbar muscle strain, chronic bronchitis, chronic hepatitis, etc. It is the main raw material for the production of various Chinese patent medicines. It is mainly produced in Guangdong, Guangxi, Hainan and Vietnam in my country. The leaves, flowers, fruits and tubers of different provenances have different shapes and growth performances. The root system has strong penetrating power a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G22/25A01G31/00
CPCA01G22/00A01G24/00A01G31/00A01H4/001A01H4/008
Inventor 黄妹兰
Owner 台州中知英健机械自动化有限公司
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