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Microfluidic chip, analysis device and analysis method based on microfluidic chip

A technology of microfluidic chip and microfluidic channel, which is applied in the direction of measuring devices, analytical materials, chemical instruments and methods, etc., which can solve the problems of low purity and recovery rate, destruction of exosomes, reduction of efficiency and sensitivity of flow cytometry, etc. problem, to achieve the effect of simple and convenient operation

Active Publication Date: 2020-06-09
SHENZHEN HUIXIN LIFE TECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are many problems in using PEG to precipitate exosomes: such as low purity and recovery rate, more impurities, uneven particle size, and polymers that are difficult to remove. In addition, the physical effects used in PEG-base such as mechanical force or Tween- Chemical additives such as 20 will destroy exosomes, etc., so it is easy to be questioned
For exosomes, which are much smaller in size than cells, the efficiency and sensitivity of flow cytometry will be greatly reduced

Method used

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  • Microfluidic chip, analysis device and analysis method based on microfluidic chip
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Embodiment Construction

[0033] In order to have a clearer understanding of the technical features, purposes and effects of the present invention, the specific implementation manners of the present invention will now be described in detail with reference to the accompanying drawings.

[0034] A tumor as used in the present invention is defined as a mass of abnormally growing cells. Tumors useful for the present invention include, but are not limited to, prostate cancer, breast cancer, lung cancer, colon cancer, gastric cancer, endometrial cancer, liver cancer, esophageal cancer, bladder cancer, oral cancer, thyroid cancer, pancreatic cancer, retinal and skin cancer, preferably for pancreatic cancer.

[0035] "Tumor sample" refers to a sample taken from a patient before or after resection of the tumor.

[0036] In the present invention, "liquid sample" is called "tumor sample", and "liquid sample" can be isolated from any tissue, cell, cell extract, saliva, urine, serum, whole blood, plasma concentrat...

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PUM

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Abstract

The invention relates to a microfluidic chip, which is provided with a microfluidic channel, a filter element and a detection area, and the filter element is arranged in the microfluidic channel, and is used for filtering a liquid sample so that the liquid containing target particles flows into the detection area. area. The present invention also relates to an analysis device based on a microfluidic chip, which includes the above-mentioned microfluidic chip and a detection and analysis unit, the detection and analysis unit is used to detect and analyze the detection area to obtain the biological information of the target particle. In addition, the present invention relates to a method for analyzing target particles in said liquid sample.

Description

technical field [0001] The invention relates to a microfluidic chip and its application, belonging to the field of biological detection. Background technique [0002] Biological samples, such as exosomes, can carry a variety of proteins, mRNA, and miRNA, and participate in processes such as cell communication, cell migration, angiogenesis, and tumor cell growth. At present, the detection methods of exosomes mainly include enzyme-linked immunosorbent assay (ELISA) and flow cytometry. flow cytometer. [0003] Enzyme-linked immunosorbent assay has low sensitivity, time-consuming and cumbersome operation, which has certain limitations in the detection of exosomes. [0004] Ultracentrifugation is currently the most commonly used method for exosome purification. The number of exosomes obtained by simple operation is large, but the process is time-consuming and the recovery rate is unstable, and the purity is also questioned. cause damage, thereby reducing its quality. [0005]...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01L3/00G01N33/574
CPCB01L3/5027B01L2200/10G01N33/57488
Inventor 陈欲超
Owner SHENZHEN HUIXIN LIFE TECH CO LTD
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