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Extraction for polyphenols from honey made by stingless bees and applications of extract in immunoregulation

A technology of honey extract and stingless bee, applied in the field of biochemistry, to achieve significant anti-oxidation and wide application value

Active Publication Date: 2017-12-05
BEE RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But there is no research report on the composition and activity of stingless bee honey in my country

Method used

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  • Extraction for polyphenols from honey made by stingless bees and applications of extract in immunoregulation
  • Extraction for polyphenols from honey made by stingless bees and applications of extract in immunoregulation
  • Extraction for polyphenols from honey made by stingless bees and applications of extract in immunoregulation

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Experimental program
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Effect test

Embodiment 1

[0032] Weigh 800g of stingless bee (Hainan, Trigona ventralis Swith) honey, dissolve it in 4L acetic acid (pH 2.0) water, stir and dissolve, sonicate for 30min, filter with cotton, and set aside. After soaking Amberlite XAD-2 macroporous resin in ethanol for 24 hours, weigh 800g of activated macroporous resin, wash it with water 2-3 times until it is odorless, pour it into the aqueous solution of stingless bee honey, and stir for 1 hour to absorb. Stand still, discard the supernatant, fill the macroporous resin into a glass column, rinse with 1.6L pH 2 acetic acid aqueous solution, 2.4L pure water to remove polar substances such as sugar, and finally use 6.4L anhydrous The ethanol solution was eluted and the eluate was collected, concentrated under reduced pressure at 40°C to a solid, a total of 1.2152 g, redissolved in 10 mL of pure water, and extracted with 40 mL of ethyl acetate. The ethyl acetate layer was collected and blown dry with nitrogen to obtain 0.552 g of stingles...

Embodiment 2

[0038] Example 2 Test of Immunomodulatory Ability of Stingless Bee Honey Extract

[0039] Remarkable reproducibility and Reliability, widely used to quantify the antioxidant capacity of natural products and plant extracts. DPPH is a very stable nitrogen-centered free radical, which reflects its antioxidant properties by capturing free radicals of substances; ABTs+ is generated after oxidation of ABTS, and ABTs+ is inhibited when antioxidants exist; reducing Fe 3+ The ionic capacity can reflect the antioxidant capacity of the substance. Stingless Bee Honey Extract DPPH IC 50 The value is 435±0.54 μg / mL, ABTS IC 50 The value is 423±0.34μg / mL, RP is 0.582±0.02μmol Trolox / g, and has good oxidation resistance.

[0040] Table 2 Contents of total phenolic acids and total flavonoids and antioxidant capacity of stingless bee honey

[0041]

[0042]

[0043] Macrophages play a key role in innate immunity, and activated macrophages can initiate and increase a variety of inflam...

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Abstract

The invention provides a method for extracting polyphenols from honey made by stingless bees. The method comprises the following steps: (1) dissolving honey made by stingless bees by adopting acetic acid water solution, carrying out stirring and ultrasonic treatment, and carrying out filtering, thus separating out water solution of the honey made by the stingless bees; (2) pouring macroporous resin into the water solution of the honey made by stingless bees, and carrying out stirring for adsorbing for 0.5-2 h; (3) packing the macroporous resin after adsorption into a column, carrying out leaching by adopting acetic acid water solution and pure water, and finally, carrying out elution by adopting alcohol solution; and (4) carrying out reduced pressure vacuum concentration on the eluate till solid matter is formed, re-dissolving the solid matter in pure water, and carrying out extraction by adopting ethyl acetate. According to the technical scheme, the active ingredients of the honey made by the stingless bees are extracted and enriched through XAD-2 column chromatography, the ingredient analysis and application research for the extract of the honey made by the stingless bees are carried out, the result shows that the extract of the honey made by the stingless bees contains rich active ingredients such as flavone and polyphenols, and has the obvious oxidation and inflammation resisting activity, and thus the extract has a wide application value for the development and application of medicines, foods and health products for preventing and treating inflammatory diseases.

Description

technical field [0001] The invention belongs to the field of biochemistry, and in particular relates to a honey extraction method and an extraction product. Background technique [0002] Stingless bees are a class of insects that live in groups and can make honey, belonging to Hymenoptera, Apoidea, Apidae, Apinae, wheat Bee family (MeliPonini), stingless bee (Trigona Jurine), black body, small and flexible, is the main pollinator species of tropical plants, mainly distributed in tropical and subtropical regions, my country is only in Yunnan, Hainan and Taiwan The field forests are distributed. Stingless bee honey is nectar collected by stingless bees from plant nectaries or other parts. After full brewing, it is a sweet substance stored in the nest room. Compared with Western bee honey, it has high moisture content, high acidity and low enzyme value. , Low viscosity and so on. Honey has long been recognized as a natural sweetener and is also widely used in clinical practice...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/644A61P37/02A61P29/00
CPCA61K35/644
Inventor 王凯梁馨文王亮吴黎明薛晓锋
Owner BEE RES INST CHINESE ACAD OF AGRI SCI
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