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Detection model for mouse intestinal mucosa epithelial barrier function and intestinal mucosa immune tolerance cell

A technology of immune tolerance and detection models, which is applied in the direction of measuring devices, particle and sedimentation analysis, biochemical equipment and methods, etc., can solve the problems of not establishing detection indicators, etc., and achieve the effect of inhibiting allergic reactions

Inactive Publication Date: 2017-11-24
SHENZHEN UNIV
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Problems solved by technology

[0003] In summary, the problems in the prior art are: the intestinal immune tolerance system plays a vital role in maintaining the health and function of the intestinal mucosa; the prior art has not established relevant detection indicators

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  • Detection model for mouse intestinal mucosa epithelial barrier function and intestinal mucosa immune tolerance cell
  • Detection model for mouse intestinal mucosa epithelial barrier function and intestinal mucosa immune tolerance cell
  • Detection model for mouse intestinal mucosa epithelial barrier function and intestinal mucosa immune tolerance cell

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Embodiment Construction

[0021] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0022] The application principle of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0023] The mouse intestinal mucosal epithelial barrier function and intestinal mucosal immune tolerance cell detection model provided by the embodiment of the present invention is as follows: a part of Gal-1-deficient mice are intraperitoneally injected with Gal-1, 0.25 mg / mouse, once a day, for 7 days in total ; Small intestines were obtained from Gal-1-deficient mice, B6 mice and mice injected with Gal-1, respectively.

[0024] Such as figure 1 As shown, the establishment method of...

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Abstract

The invention belongs to the technical field of body immunity, and discloses a detection model for a mouse intestinal mucosa epithelial barrier function and an intestinal mucosa immune tolerance cell. The model is characterized in that one part of Gal-1 deficient mice is subjected to Gal-1 intraperitoneal injection, and each mouse is injected with 0.25mg each time per day, and injection is carried out for 7 days; small intestines are respectively taken from the Gal-1 deficient mice, B6 mice and mice injected with Gal-1. The method comprises the following steps: obtaining the Gal-1 deficient mice, wherein the Gal-1 deficient mice are subjected to the Gal-1 intraperitoneal injection; respectively taking the intestines from the Gal-1 deficient mice, the B6 mice and the mice injected with Gal-1, and installing in a Ussing chamber system; measuring an epithelial barrier function; taking the small intestines to separate LPMC (Lamina Propria Mononuclear Cells), and detecting immune tolerance cells. The Gal-1 has an immunoregulation function, the intestinal mucosa is an important organ for forming oral tolerance, and the IEC (Intestinal Epithelial cCells) has an immune tolerance function.

Description

technical field [0001] The invention belongs to the technical field of body immunity, and in particular relates to a mouse intestinal mucosal epithelial barrier function and intestinal mucosal immune tolerance cell detection model. Background technique [0002] Immune tolerance refers to the specific immune non-response state produced by the body's immune system after accepting the action of a certain antigen. Individuals who have developed immune tolerance to a specific antigen do not develop an immune response or cannot develop an immune response that can be detected by conventional methods after being stimulated by the antigen again. Therefore, the normal immune tolerance mechanism is very important to maintain the health of the body. The mechanism of immune tolerance is still not very clear. Although the international and domestic research on the factors that impair immune tolerance has made rapid progress in recent years, the restoration of the established immune tole...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02G01N15/14A61K38/17
CPCG01N15/14A61K38/1732G01N33/505G01N33/5052
Inventor 杨平常杨贵徐灵芝谢瑞娣刘志强
Owner SHENZHEN UNIV
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