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Astragalus seed protein and application thereof to foods having function of resisting sport fatigue

A technology for astragalus seeds and exercise fatigue is applied in the research field of natural products and their functions to achieve the effects of easy industrialization, popularization and application, mature extraction and separation methods, and rich protein content.

Active Publication Date: 2017-11-07
UNIVERSITY OF MACAU +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are few reports on the related research on extracts from Astragalus seeds, but there is no report on the anti-exercise fatigue effect of Astragalus seed protein

Method used

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  • Astragalus seed protein and application thereof to foods having function of resisting sport fatigue
  • Astragalus seed protein and application thereof to foods having function of resisting sport fatigue
  • Astragalus seed protein and application thereof to foods having function of resisting sport fatigue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A method for extracting and separating astragalus seed protein, comprising the following steps:

[0030] (1) Crush the Astragalus seeds, degrease with petroleum ether first, then add KH 2 PO 4 -NaOH buffer solution was stirred and leached for 6-12h, centrifuged, and the supernatant was taken; the above-mentioned leaching operation was repeated 2-3 times, and the obtained supernatant was combined to obtain the extract;

[0031] (2) the extract is slowly added powdered ammonium sulfate to a saturation of 30% under constant stirring, centrifuged, and the supernatant is collected; the supernatant is slowly added to a saturation of 40% under constant stirring, Centrifuge to collect the precipitate;

[0032] (3) Transfer the precipitate to a dialysis bag, dialyze in deionized water (change the water every 24 hours on average) until the dialysate is colorless, and finally freeze-dry the components in the dialysis bag to obtain astragalus seed protein.

Embodiment 2

[0034] A method for extracting and separating Astragalus seed protein is to adopt ammonium sulfate saturated precipitation method, which also includes 3 steps, and its steps (1) and (3) are the same as in Example 1, and step (2) is as follows:

[0035] (2) the extract is slowly added powdered ammonium sulfate to 40% saturation under constant stirring, centrifuged to collect the supernatant; the supernatant is then slowly added powdered ammonium sulfate to 60% saturation under constant stirring, Centrifuge to collect the precipitate.

Embodiment 3

[0037] A method for extracting and separating Astragalus seed protein is to adopt ammonium sulfate saturated precipitation method, which also includes 3 steps, and its steps (1) and (3) are the same as in Example 1, and step (2) is as follows:

[0038] (2) the extract is slowly added powdered ammonium sulfate to 60% saturation under constant stirring, centrifuged to collect the supernatant; the supernatant is then slowly added powdered ammonium sulfate to 80% saturation under constant stirring, Centrifuge to collect the precipitate.

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Abstract

The invention discloses an astragalus seed protein and the application of the astragalus seed protein to foods having a function of resisting sport fatigue. An extracting and separating method of the protein comprises the following steps: pulverizing astragalus seeds, degreasing with petroleum ether, adding a KH2PO4-NaOH buffer solution, stirring, extracting for 6-12 hours to obtain an extracting solution; adding powdery ammonium sulfate into the extracting solution to the saturability of 30%, centrifuging and collecting a supernate; adding the powdery ammonium sulfate to the saturability of 80%, centrifuging and collecting a precipitate; dialyzing the precipitate until a dialysate is colorless, and finally freeze-drying to obtain the astragalus seed protein. The astragalus seed protein is prepared through an ammonium sulfate saturated precipitation method, and the saturability of the ammonium sulfate is 30-80%. The astragalus seed protein can be added into a biscuit, a liquid drink or a powder drink used as a nutritional supplement for resisting sport fatigue, is convenient to use, and meets sports people liking different food types.

Description

technical field [0001] The invention belongs to the field of research on natural products and their functions, and in particular relates to astragalus seed protein and its application in functional food for resisting sports fatigue. Background technique [0002] When the athlete's training and competition load exceeds the body's ability to bear, it will produce a temporary decline in physiological function. When the body is exercising, the physiological process cannot continue to maintain its function at a specific level or cannot maintain a predetermined exercise intensity, resulting in physical discomfort, which is called exercise-induced fatigue. Relieve sports fatigue, in the field of competitive sports, it can avoid or reduce sports injuries caused by excessive exercise and extreme sports; in the field of mass fitness, it can avoid metabolic disorders caused by excessive exercise fatigue, and avoid endocrine and internal organ tissues. damage. [0003] Common methods ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C07K1/34A23L33/185A23L2/39A23L2/52A21D13/064
CPCA21D13/06A23L2/39A23L2/52A23L33/185A23V2002/00C07K14/415A23V2200/30A23V2250/548
Inventor 索思卓徐健李续娥徐晓阳胡豪
Owner UNIVERSITY OF MACAU
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