Molecular marker and method for rapid breeding of high quality erect panicle rice variety

A molecular marker and high-quality technology, which is applied in the fields of botany equipment and methods, biochemical equipment and methods, and microbial measurement/inspection, can solve the problems of time-consuming and labor-intensive breeding methods, inaccurate identification of erect panicle traits, etc., and achieve shortening Effects of breeding cycles, accurate yield potential, increased efficiency

Inactive Publication Date: 2017-10-20
RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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Problems solved by technology

[0004] The purpose of the present invention is to overcome the deficiencies in the prior art, and provide a molecular marker and method for rapid selection of high-quality erect panicle rice varieties, so as to overcome the inaccurate identification of traditional erect panicle traits and time-consuming breeding methods for high-quality erect panicle varieties Technical problems such as effort

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  • Molecular marker and method for rapid breeding of high quality erect panicle rice variety
  • Molecular marker and method for rapid breeding of high quality erect panicle rice variety
  • Molecular marker and method for rapid breeding of high quality erect panicle rice variety

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Embodiment 1

[0028] 1. Materials

[0029] The methods used in this example are conventional methods known to those skilled in the art unless otherwise specified, and the materials such as reagents used are commercially available products unless otherwise specified, and the germplasm resources involved are, unless otherwise specified Note, all come from China National Germplasm Bank.

[0030] 2. Method

[0031] 2.1 Acquisition of Erect Ear Varieties Containing the Molecular Markers

[0032] Radiation mutagenesis was carried out on Nongyuan 238, and a mutant F190 with an erect panicle phenotype was found. Compared with the wild type, the mutant had shorter plant height, shorter panicle length and grain shape, denser kernels, and a more compact plant shape. , the yield per plant decreased not significantly. Further using the map-based cloning method, it was found that the gene controlling the erect panicle phenotype was allelic with the EP2 gene. In F190, the EP2 gene had a single base G m...

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Abstract

The invention discloses a molecular marker and a method for rapid breeding of a high quality erect panicle rice variety, the molecular marker is rice erect panicle gene EP2 of which a single base G at the connection position of the sixth intron and the seventh exon is mutated into A, and the method is as follows: development of a dCAPs marker primer by use of the molecular marker and rapid breeding of the high quality erect panicle rice variety by a method of combination of molecular marker assisted selection and traditional breeding. The molecular marker has polymorphism between F190 and a plurality of curved panicle parents, The marker primer EP2- dCAP-s-1 can distinguish a differential fragment between the erect panicle donor parent F190 and the curved panicle parents, compared with the molecular marker in the prior art, a required fragment can be accurately and quickly introduced in, objectives are clear, a breeding cycle is shortened, cost is saved, efficiency of selection is effectively improved, and an erect panicle variety with high yield potential can be bred more quickly and more accurately.

Description

technical field [0001] The invention relates to the technical field of crop biological breeding, in particular to a molecular marker for rapid selection of high-quality erect panicle rice varieties and a method thereof. Background technique [0002] Rice is the most important food crop in my country. With the improvement of people's living standards, the demand for rice is increasing. Due to the limitation of arable land area, it is particularly important to increase the yield of rice. Panicle shape is one of the important factors affecting rice yield, and improving the panicle shape structure can help increase rice yield. In japonica rice breeding, the selection of curved panicle varieties to erect panicle varieties is an important transition. As early as the early 1980s, a breeding model for cultivating erect panicle japonica varieties was proposed. The erect panicle varieties are conducive to light energy utilization. In recent years, breeders have bred a large number of...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11A01H1/02
CPCC12Q1/6895A01H1/02C12Q2600/13C12Q2600/156
Inventor 周坤能李泽福夏加发王元垒马廷臣
Owner RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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