Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of NK cells

A technology of NK cells and nuclear cells, applied in the field of bioengineering, can solve the problems of non-standardized process in the culture process and difficulty in realizing large-scale culture of NK cells.

Inactive Publication Date: 2017-10-20
湖南开启时代生物科技有限责任公司
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the in vitro culture of NK cells is only carried out by using the culture medium in a petri dish. The culture process is not standardized, and it is difficult to achieve large-scale culture of NK cells.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of NK cells
  • Preparation method of NK cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0032] The embodiment of the present invention discloses a preparation method of NK cells, which uses a separation culture tank to prepare NK cells, and the steps are standardized, which facilitates the large-scale production of NK cells.

[0033] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0034] see Figure 1-Figure 2 , the embodiment of the present invention provides a method for preparing NK cells, which is used for fully enclosed NK cell preparation equipment, which includes:

[0035] 1. Add the blood sa...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparation method of NK cells. The preparation method of the NK cells comprises the following steps: (1) adding a blood sample and an isovolumetric lymphocyte separation medium in a separation culture tank for centrifugal separation so as to obtain single karyocytes; (2) sorting the single karyocytes to purify NK cells; (3) carrying out induced culture on the NK cells in the separation culture tank; (4) carrying out amplification culture on the NK cells in the separation culture tank to obtain an NK cell final product, and 5) washing packing the NK cell final product. In the preparation method of the NK cells, the NK cells are prepared by the separation culture tank. Compared with a mode of culturing the NK cells in vitro by using a culture dish in the prior art, the preparation method of the NK cells is standard in steps, and large-scale production of the NK cells is realized conveniently.

Description

technical field [0001] The invention relates to the technical field of bioengineering, and more specifically, relates to a preparation method of NK cells. Background technique [0002] NK (natural killer, natural killer) cells are important immune cells in the body, not only related to anti-tumor, anti-virus infection and immune regulation, but also involved in the occurrence of hypersensitivity and autoimmune diseases in some cases. [0003] At present, the in vitro culture of NK cells is only carried out by using the culture medium in a petri dish. The culture process is not standardized, and it is difficult to achieve large-scale culture of NK cells. [0004] To sum up, how to provide a preparation method suitable for NK cells so as to facilitate the large-scale production of NK cells is a technical problem to be solved urgently by those skilled in the art. Contents of the invention [0005] In view of this, the present invention provides a method for preparing NK cell...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0783
CPCC12N5/0646C12N2509/00
Inventor 赵明张锴宋志兵刘肖琳
Owner 湖南开启时代生物科技有限责任公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com