Method for improving drought resistance of citruses
A drought-resistant, citrus technology, applied in the fields of botanical equipment and methods, chemicals for biological control, agricultural gas emission reduction, etc., to achieve the effect of promoting vegetative growth, low production cost, and improving the stability of aggregates
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Embodiment 1
[0027] 1. Preparation of glomycin protein drought-resistant agent
[0028] Collect the yellow soil with soil depth of 0-20cm from the orange orchard. This embodiment adopts the 0-20cm soil (yellow soil) collected in March, 2016 in the 26-year-old citrus orchard of Changjiang University, Jingzhou City. Sieve to remove roots, litter, and rocks from the soil. Then repeatedly extract a sufficient amount of easy-to-extract glomycin solution according to the following method:
[0029] Weigh 5.0g of the treated soil and put it into a centrifuge tube, add 40mL of 20mmol / L (pH=7.0) sodium citrate extract, then shake on the shaker for 60s, high temperature sterilization pot 103kpa, 121℃ Extract for 30 minutes, centrifuge at 10,000 g / s for 10 minutes, collect the supernatant to obtain an easily extractable glomycin solution, and measure the concentration of the easily extractable glomycin in the soil to be 0.014 mg / mL after the extraction is completed. Finally, use the complete easy-to...
Embodiment 2
[0071] This embodiment provides a kind of anti-drought agent that improves the drought resistance of citrus, and its preparation step comprises:
[0072] S1. Collect 0-20cm yellow soil in the orangery, mix evenly, air-dry, pass through a 4mm sieve to remove impurities such as roots, garbage and stones in the soil;
[0073] S2, prepare 20mmol / L (pH=7.0) sodium citrate extract: (1) A solution: 0.02mol / L citric acid solution (take 4.2g C 6 h 8 o 7 ·H 2 O dissolved in 1000mL distilled water); (2) B solution: 0.02mol / L disodium hydrogen phosphate solution (take 5.365gNa 2 HPO 4 ·7H 2 O or 7.16g Na 2 HPO 4 12H 2 O dissolved to 1000mL); (3) Measure 130mL and 870mL of A liquid and B liquid respectively, mix well to obtain 1000mL 20mmol / L, pH=7.0 sodium citrate buffer.
[0074] Take 4.0g of the soil obtained in step S1 and put it into a 50mL centrifuge tube, add 32mL of 20mmol / L, pH=7.0 sodium citrate buffer, shake for 60s, then extract at 103kpa, 121°C for 30min, and then ext...
Embodiment 3
[0077] This embodiment provides a kind of anti-drought agent that improves the drought resistance of citrus, and its preparation step comprises:
[0078] S1. Collect 0-20cm yellow soil in the orangery, mix evenly, air-dry, pass through a 4mm sieve to remove impurities such as roots, garbage and stones in the soil;
[0079] S2, prepare 20mmol / L (pH=7.0) sodium citrate extract: (1) A solution: 0.02mol / L citric acid solution (take 4.2g C 6 h 8 o 7 ·H 2 O dissolved in 1000mL distilled water); (2) B solution: 0.02mol / L disodium hydrogen phosphate solution (take 5.365gNa 2 HPO 4 ·7H 2 O or 7.16g Na 2 HPO 4 12H 2 O dissolved to 1000mL); (3) Measure 130mL and 870mL of A liquid and B liquid respectively, mix well to obtain 1000mL 20mmol / L, pH=7.0 sodium citrate buffer.
[0080] Take 5.0g of the soil obtained in step S1 and put it into a 50mL centrifuge tube, add 32mL of 20mmol / L, pH=7.0 sodium citrate buffer, shake for 60s, then extract at 103kpa, 121°C for 30min, and then extra...
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Abstract
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