Preparation and application methods of efficient diluent for giant salamander sperm

Abstract

The invention discloses a preparation and application methods of an efficient diluent for giant salamander sperm and relates to the technical field of giant salamander cultivation methods. The efficient diluent is prepared by medical 0.9% sodium chloride injection, double distilled water, glycerin, glucose, sodium citrate and mycillin. During use, the diluent is used to dilute the sperm, the diluted sperm is used to perform insemination on eggs, and the eggs are submerged by the diluted sperm in a way that the diluted sperm is 1cm higher than the eggs during the insemination. The preparation and application methods have the advantages that the giant salamander sperm is diluted by the combined diluent, the swimming range of sperms in a bath solution is expanded, the bonding surfaces of the sperms and the eggs is enhanced greatly, the user amount of pure sperm in each insemination is reduced, the sperms with low activity cannot swim into egg envelopes to inseminate the eggs, malformed or weak residual bodies are reduced greatly, survival of the fittest is achieved before the insemination, and the quality of the insemination is increased effectively.

Description

Technical field: [0001] The invention relates to a giant salamander breeding method and the technical field, in particular to a method for preparing and using a high-efficiency diluent for giant salamander semen. Background technique: [0002] The giant salamander is the largest and most precious amphibian in existence in the world. Its cry is very similar to the cry of a baby, so people also call it "baby fish". The giant salamander is a national second-class protected amphibious wild animal. It is a key development of agricultural industrialization and characteristic agriculture and a species of wild animal genetic protection. Female salamanders lay eggs every July to August. The eggs are laid in rock caves, and each fish lays more than 300 eggs. The rest of the rearing tasks are left to the male salamanders. The male salamander bends its body into a semicircle and surrounds the eggs to avoid being washed away by water or being attacked by predators. After 2 to 3 weeks, ...

AI Technical Summary

Problems solved by technology

[0003] In order to increase the production of giant salamanders, the technology of artificial breeding of giant salamanders has been promoted. However, the sperm of giant salamanders is sticky and difficult to flow. During the fertilization process, due to the small flow range, the bonding area with the egg becomes smaller, so that the semen in the fertilization process There will be a problem of repeated fertilization, resulting in the combination of weak sperm and eggs, resulting in the fertilization of weak and weak residual bodies, and the hatched juvenile salamanders are in poor health