Method for detecting non-lactoprotein in milk based on gel electrophoresis and chemometrics

Abstract

The invention belongs to the technical field of food safety detection and particularly relates to a method for detecting non-lactoprotein in milk based on gel electrophoresis and chemometrics. The method includes: using real milk and to-be-detected milk samples as the research objects, and using the SDS-PAGE protein detection technology to collect the gel map of the samples; then using the chemometrics to obtain the global visual main component score plot of the real milk and the to-be-detected milk samples so as to detect the non-lactoprotein in non-targeted milk. The method is low in cost, simple and fast to operate, stable in result and high in accuracy and has potential application and popularization prospect in milk quality detection.

Description

technical field [0001] The invention belongs to the technical field of food safety detection, and in particular relates to a method for detecting non-dairy protein in milk based on gel electrophoresis and chemometrics. Background technique [0002] Food safety is closely related to human life and is a topic of widespread concern. Among them, food adulteration is one of the important aspects leading to food safety problems. Cow's milk is widely favored by consumers because of its rich nutritional value. However, driven by economic interests, adding fake protein with low value and low cost to milk has become one of the main ways of milk adulteration. The purpose of adulterating other non-milk proteins is to increase the protein content of milk, but other structures and different types of proteins will be introduced in the process. Therefore, although chromatography, mass spectrometry and spectroscopic techniques can be used to achieve the purpose of detection, however, due t...

AI Technical Summary

Problems solved by technology

The purpose of adulterating other non-milk proteins is to increase the protein content of milk, but other structures and different types of proteins will be introduced in the process. Therefore, although chromatography, mass spectrometry and spectroscopic techniques can be used to achieve the purpose of detection, however, due to their relatively high The cost and the requirements for complex professional equipment limit the value of its use

Moreover, in some economically underdeveloped areas, due to the lack of detection resources, the experimental conditions required for chromatography, mass spectrometry and spectroscopy cannot be realized.

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