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A kind of medicinal bletilla striata tissue culture seedling propagation method

A tissue culture and tissue culture seedling technology, applied in the field of plant reproduction, can solve the problems of inability to meet the needs of large-scale planting, high variation ratio, weak seedlings, etc., and achieve the effect of satisfying large-scale planting and efficient seedling breeding.

Active Publication Date: 2019-11-22
江苏东郁植物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although the method for quickly cultivating Bletilla striata seedlings is relatively neat, the protocorms degenerate rapidly, have a high variation ratio, the seedlings are weak, and the transplanting survival rate is low, which cannot meet the needs of large-scale planting.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1: A method for breeding medicinal Bletilla striata tissue culture seedlings, comprising the following cultivation steps: S1: Explant selection, selecting the tissue culture seedlings that germinate and take root from sterile naked embryos as explants, and keeping the explants in the Sterile state; S2: Proliferation culture, part or all of the leaves and roots of the explants in the sterile state were excised, inoculated in solid proliferation medium, entered the dark culture stage for 2 days, and then entered the light culture stage for 8 weeks of proliferation , the proliferation rate is controlled at 3 times, and the multiplication obtains the buds;

[0047] S3: Strong seedling cultivation, every 12 weeks, divide the buds cultivated in the multiplication stage into single buds, and cut off part or all of the leaves, transfer them to the pre-made strong seedling medium, culture in dark for 2 days, and then light Cultivate for 2 weeks; S4: rooting induction,...

Embodiment 2

[0070] Embodiment 2: A method for breeding medicinal Bletilla striata tissue culture seedlings, the difference from Example 1 is that S2: Proliferation culture, part or all of the leaves and roots of the explants in the sterile state are excised, and inoculated on solid In the proliferation medium, keep the temperature at 25°C, enter the dark culture stage for 2 days, and then enter the light culture stage for 8 weeks. The light intensity is controlled at 1500Lux, the photoperiod is 12 / 8h, and the proliferation rate is controlled at 3 S3: Cultivation of strong seedlings. Every 12 weeks, divide the buds cultivated in the multiplication stage into single buds, cut off part or all of the leaves, and transfer the buds to the medium for strong seedlings. Maintain the temperature at 28°C, culture in the dark for 2 days, and cultivate in the light for 8 weeks. The light intensity is controlled at 2000 Lux, and the photoperiod is 12 / 8h. Take the healthy single buds as the tissue cultur...

Embodiment 3

[0071] Embodiment 3: A kind of medicinal Bletilla striata tissue culture seedling breeding method, the difference with embodiment 1 or 2 is that, S2: Proliferation culture, part or all of the leaves and roots of the explants in the aseptic state are excised, and inoculated In the solid proliferation medium, keep the temperature at 25°C, enter the dark culture stage for 7 days, and then enter the light culture stage for 16 weeks of proliferation and culture. The light intensity is controlled at 3000 Lux, the photoperiod is 20 / 8h, and the proliferation rate is controlled. At 3 times, proliferate to obtain cluster buds; S4: cultivate strong seedlings, repeat S3, remove part or all leaves of the cut cluster buds, and transfer the cluster buds to strong seedling medium, maintain the temperature at 28 °C, and keep the temperature in the dark Cultivate for 7 days, light culture for 16 weeks, light intensity is controlled at 3000Lux, photoperiod is 20 / 8h, take the single bud that grows...

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Abstract

The invention discloses a tissue culture and seedling propagation method for medicinal bletilla and aims to provide a tissue culture and seedling propagation method for medicinal bletilla with advantages of high propagation speed, high quality, suitableness for large-scale seedling propagation and the like. According to key points of the technical scheme, the method comprises the following culture steps: S1, selecting explants; S2, performing propagation culture; S3, performing strong seedling culture; S4, rooting induction. According to the previous steps, the bottlenecks that the medicinal bletilla is low in seed germination rate, difficult in seedling cultivation, low in propagation speed can be overcome; seedling propagation can be rapidly and efficiently performed at high quality, and large-scale planting needs are met, so that large-scale industrialized cultivation of the bletilla can be realized.

Description

technical field [0001] The invention relates to the technical field of plant propagation, in particular to a method for breeding medicinal bletilla striata tissue culture seedlings. Background technique [0002] Bletilla striata is a species of Bletilla striata in the family Orchidaceae, also known as Baiji. Perennial herb, 20-50 cm high, with 4-5 leaves, the bases are nested into stems, and the scapes are drawn out from the center. Racemes with several flowers; flowers purple or light red, about 5 cm in diameter, composed of 3 sepals, 2 petals and 1 specialized lip; lip 3-lobed with longitudinal pleats; stamens and The styles are conjoined to form a zygote. There is an anther at the top of the gynocolum, and a stigma recess on the upper front. There are thick rhizomes underground, like a chicken head, rich in viscosity, containing white and gum, that is, white and mannan, which can be used for medicine. paste. [0003] Bletilla striata has gorgeous flowers and is used n...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 胡春宏王晨常苹王婷婷季翔
Owner 江苏东郁植物科技有限公司
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