Cultivation method of trifolium repens
A cultivation method and white clover technology, applied in the field of white clover cultivation, can solve the problems of poor drought resistance, large water demand, rare addition combination, etc., and achieve the effects of alleviating growth retardation and improving drought resistance.
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[0050] Test material: Drought-sensitive 'Latinuo' white clover was used as the test material.
[0051] Material cultivation: The seeds are sterilized with 0.1% mercuric chloride for 5 minutes, rinsed with distilled water for 4 times and then used for later use. 0.1g of the sterilized seeds are evenly sprinkled in a seedling tray covered with quartz sand with a length of 35cm, a width of 25cm, and a height of 10cm, pour deionized water and place the seedling tray in an incubator (23 / 19°C, day / night ;75% relative humidity; 300μmol·m -2 ·s -1 The light intensity) allows the seeds to germinate for a period of 7 days, and the deionized water is changed every day. After the seeds have germinated, pour out all the deionized water and replace it with Hoagland's nutrient solution for cultivation, replace the nutrient solution every other day, and continue to cultivate for 23 days (2 mature leaves) to select materials with consistent growth for the test .
[0052] Experimental desig...
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