Method of utilizing SSR label to identify Ping'ou hybridized hazel species

Abstract

The invention discloses a method of utilizing a SSR label to identify a Ping'ou hybridized hazel species. An SSR label is combined with a capillary electrophoresis technology to establish a Ping'ou hybridized hazel species identification method based on four pairs of EST-SSR labels. For the first time, in domestic, the SSR amplification data of 43 Ping'ou hybridized hazel species (lines) widely planted in China is reported; a comparable data result is provided for the identification of unknown species; the provided method has the advantages of co-dominance, good repeatability, strong site specificity, and digitalization of amplification strips, and the method is universal, convenient and effective.

Description

technical field [0001] The invention relates to a Pingou hybrid hazel variety identification technology, in particular to a method for identifying Pingou hybrid hazel varieties using SSR markers. Background technique [0002] Pingou hybrid hazel (Corylus heterophylla Fisch.×Corylus avellana L.) is a species obtained by domestic experts in the 1980s by interspecies crossing between Chinese puffy hazel (C.heterophylla Fisch.) and foreign hazelnut (C.avellana L.). Over the past 30 years, more than 10 varieties and more than 20 excellent lines have been approved and screened. They have the characteristics of large fruit, high yield, high kernel yield, full kernel, and strong adaptability. They have been widely introduced to the All over the country, at present, the regional layout of the main varieties (strains) of Pingou hybrid hazel has been initially formed, and the national cultivation area is nearly 900,000 mu. [0003] With the rapid development of Pingou hybrid hazelnut ...

AI Technical Summary

Problems solved by technology

[0017] (1) RAPD, ISSR and AFLP markers are all dominant markers, the number of amplified fragments is large, and false positive fragments are prone to exist, resulting in reduced identification accuracy;

[0018] (2) The difference between samples is distinguished by the relative position of the amplified bands and the clustering diagram generated, and the size of the bands is not digitized;

[0019] (3) The number of Pingou hybrid hazel varieties (strains) involved is limited;

[0020] (4) Variety identification methods are different in different laboratories and different batches of experiments, or rely on control varieties, the method is not universal and convenient

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