A kind of lactic acid bacteria compound microbial agent and its application for improving the yield traits of saline-alkali ground peanut

A compound bacterial agent and lactic acid bacteria technology, applied in the field of microorganisms, can solve the problems of reduced yield, small size of peanut fruit, and no effective technical means to solve the problem of peanuts in saline-alkali land, and achieve the effects of quality improvement, quality and yield traits.

Active Publication Date: 2020-05-05
金华银河生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the poor soil environment of saline-alkali land seriously damages the growth of peanut roots in the soil. The lack of various trace elements in the saline-alkali soil causes the growth of peanut crops to be thin and thin, and the surface of the saline-alkali soil hardens, resulting in smaller peanut fruits. , lower yield
At present, there is still no effective technical means to increase peanut production in saline-alkali land

Method used

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  • A kind of lactic acid bacteria compound microbial agent and its application for improving the yield traits of saline-alkali ground peanut
  • A kind of lactic acid bacteria compound microbial agent and its application for improving the yield traits of saline-alkali ground peanut
  • A kind of lactic acid bacteria compound microbial agent and its application for improving the yield traits of saline-alkali ground peanut

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: the preparation of compound lactic acid bacteria agent

[0021] (1) Bifidobacterium animalis subsp.Lactis V9, Lactobacillus plantarum P-8 and Lactobacillus casei Zhang were inoculated in the MRS culture medium at 6% inoculum, and Cultivate at a constant temperature of 35-37°C for 18 hours, after centrifugation and washing, add 10% sterilized skim milk emulsion, 0.6% glucose and 0.8% yeast extract to the bacterial precipitate, and adjust the number of bacteria to be greater than 2.0×10 9 cfu / mL, mix well and pour into culture respectively.

[0022] (2) Use sterile water to prepare a protective agent, consisting of: 100g / L skimmed milk powder, 30mL / L glycerin, 100g / L maltodextrin, 150g / L sea lacquer sugar and 10g / L L-sodium glutamate.

[0023] (3) Inoculate the cultures of Bifidobacterium animalis subsp.Lactis V9, Lactobacillus plantarum P-8 and Lactobacillus casei Zhang obtained in step (1) into the MRS culture respectively. cultured at a constant temperatu...

Embodiment 2

[0025] Embodiment 2: the preparation of compound lactic acid bacteria agent

[0026] (1) Inoculate Lactobacillus plantarum (Lactobacillus plantarum) P-8 and Lactobacillus casei (Lactobacillus casei) Zhang in the MRS culture medium at a 6% inoculum size, culture at a constant temperature of 35-37°C for 18 hours, and wash by centrifugation Finally, add 10% sterilized skim milk emulsion, 0.6% glucose and 0.8% yeast extract on the thalline precipitation, and adjust its bacterial count to be greater than 2.0×10 9 cfu / mL, mix well and pour into culture respectively.

[0027] (2) Use sterile water to prepare a protective agent, consisting of: 100g / L skimmed milk powder, 30mL / L glycerin, 100g / L maltodextrin, 150g / L sea lacquer sugar and 10g / L-sodium glutamate.

[0028] (3) Inoculate the cultures of Lactobacillus plantarum P-8 and Lactobacillus casei Zhang obtained in step (1) into the MRS culture medium respectively, and cultivate them at a constant temperature of 35-37°C for 18 hour...

Embodiment 3

[0030] Embodiment 3: the preparation of bifidobacterium bacterial agent

[0031] (1) Inoculate Bifidobacterium animalis subsp.Lactis V9 in the MRS culture medium at an inoculum size of 6%, and cultivate it at a constant temperature of 35-37°C for 18 hours. Add 10% sterilized skim milk emulsion, 0.6% glucose and 0.8% yeast extract, and adjust the number of bacteria to be greater than 2.0×10 9 cfu / mL, mix well and pour into culture.

[0032] (2) Use sterile water to prepare a protective agent, consisting of: 100g / L skimmed milk powder, 30mL / L glycerin, 100g / L maltodextrin, 150g / L sea lacquer sugar and 10g / L-sodium glutamate.

[0033] (3) Inoculate the cultures of Bifidobacterium animalis subsp.Lactis V9 strains obtained in step (1) into MRS culture medium respectively, cultivate them at a constant temperature of 35-37°C for 18 hours, and then inoculate them with 10% incinerated In the mixed solution of bacterial defatted milk emulsion, 0.6% glucose and 0.8% yeast extract, cult...

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Abstract

The invention provides lactobacillus complex microbial inoculants for improving saline-alkali soil peanut yield trait and application thereof. Active bacteria in the lactobacillus complex microbial inoculants are prepared from Bifidobacterium animalis subsp. Lactis V9, Lactobacillus plantarum P-8 and Lactobacillus casei Zhang. The lactobacillus complex microbial inoculants have an obvious effect on growth and metabolism of saline-alkali soil peanuts, and especially can improve quality and yield trait of the saline-alkali soil peanuts; thus, an ideal bacterial manure raw material is provided for improving saline-alkali soil peanut quality and improving saline-alkali soil peanut yield.

Description

technical field [0001] The invention relates to a lactic acid bacteria compound bacterial agent capable of significantly improving the quality and yield traits of peanuts in saline-alkali land, in particular to Bifidobacterium animalis subsp. Lactis V9, Lactobacillus plantarum P-8 and Lactobacillus casei (Lactobacillus casei) Zhang and a lactic acid bacteria compound bacterial agent composed of its metabolites belong to the technical field of microorganisms. Background technique [0002] Peanut is also known as groundnut, dicotyledonous plant, the veins of the leaves are reticular veins, and the seeds are covered with peanut peel. In history, it was called longevity fruit, ground bean, falling flower ginseng, falling pine, Chengshou fruit, fandou, fig, ground fruit, and Tang Rendou. Flowers grow in nourishment and nourishment, and help to prolong life, so the folks are also called "longevity fruit", and like soybeans, they are known as "vegetable meat" and "vegetable meat"....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/20A01P21/00C05F11/08C12R1/01C12R1/25C12R1/245
CPCA01N63/10C05F11/08C12N1/20A01N2300/00
Inventor 邹晓霞赵金山张晓军刘华伟李海梅王建林王月福王铭伦
Owner 金华银河生物科技有限公司
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