A compound lactic acid bacteria preparation and its application for improving the utilization rate of peanut micronutrients in saline-alkali land

A technology of compounding lactic acid bacteria and saline-alkali soil, applied in the field of microorganisms, can solve the problems of easy lack of trace elements, aggravating the obstacles of continuous peanut cropping, affecting the yield of peanuts, etc.

Active Publication Date: 2020-07-07
金华银河生物科技有限公司
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the availability of trace elements in the soil generally increases under acidic conditions and decreases under alkaline conditions. Due to the influence of soil conditions, saline-alkali soils are more likely to lack trace elements
At the same time, existing studies have shown that the stress of mineral nutrients caused by the lack of trace elements can aggravate the continuous cropping obstacles of peanuts and affect the yield of peanuts.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A compound lactic acid bacteria preparation and its application for improving the utilization rate of peanut micronutrients in saline-alkali land
  • A compound lactic acid bacteria preparation and its application for improving the utilization rate of peanut micronutrients in saline-alkali land
  • A compound lactic acid bacteria preparation and its application for improving the utilization rate of peanut micronutrients in saline-alkali land

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: the preparation of compound lactic acid bacteria agent

[0020] (1) Inoculate Lactobacillus plantarum P-8, Lactobacillus casei Zhang, and Lactobacillus plantarum CCFM8661 in the MRS culture medium at 6% inoculum, and inoculate at 35-37°C Cultivate at a constant temperature for 18 hours, after centrifugation and washing, add 10% sterilized skim milk emulsion, 0.6% glucose and 0.8% yeast extract to the bacterial precipitate, and adjust the number of bacteria to be greater than 2.0×10 9 cfu / mL, mix well and pour into culture respectively.

[0021] (2) The protective agent was prepared using sterile water, which consisted of: 100g / L skimmed milk powder, 30mL / L glycerin, 100g / L maltodextrin, 150g / L sea lacquer sugar and 10g / L-sodium glutamate.

[0022](3) The cultures of Lactobacillus plantarum (Lactobacillus plantarum) P-8, Lactobacillus casei (Lactobacillus casei) Zhang and Lactobacillus plantarum (Lactobacillus plantarum) CCFM8661 strain culture obtained in...

Embodiment 2

[0024] Embodiment 2: the preparation of compound lactic acid bacteria agent

[0025] (1) Inoculate Lactobacillus plantarum (Lactobacillus plantarum) P-8 and Lactobacillus casei (Lactobacillus casei) Zhang in the MRS culture medium at an inoculum size of 6%, culture at a constant temperature of 35-37°C for 18 hours, and wash by centrifugation Finally, add 10% sterilized skim milk emulsion, 0.6% glucose and 0.8% yeast extract on the thalline precipitation, and adjust its bacterial count to be greater than 2.0×10 9 cfu / mL, mix well and pour into culture respectively.

[0026] (2) Use sterile water to prepare a protective agent, consisting of: 100g / L skimmed milk powder, 30mL / L glycerin, 100g / L maltodextrin, 150g / L sea lacquer sugar and 10g / L L-sodium glutamate.

[0027] (3) Inoculate the cultures of Lactobacillus plantarum P-8 and Lactobacillus casei Zhang obtained in step (1) into the MRS culture medium respectively, and cultivate them at a constant temperature of 35-37°C for 1...

Embodiment 3

[0029] Embodiment 3: the preparation of compound lactic acid bacteria agent

[0030] (1) Inoculate Lactobacillus plantarum (Lactobacillus plantarum) CCFM8661 and Lactobacillus casei (Lactobacillus casei) Zhang in the MRS culture medium at a 6% inoculum amount, culture at a constant temperature of 35-37°C for 18 hours, and centrifuge and wash , add 10% sterilized skim milk emulsion, 0.6% glucose and 0.8% yeast extract on the thallus precipitation, and adjust the number of bacteria to be greater than 2.0×10 9 cfu / mL, mix well and pour into culture respectively.

[0031] (2) Use sterile water to prepare a protective agent, consisting of: 100g / L skimmed milk powder, 30mL / L glycerin, 100g / L maltodextrin, 150g / L sea lacquer sugar and 10g / L L-sodium glutamate.

[0032] (3) Inoculate the cultures of Lactobacillus plantarum CCFM8661 and Lactobacillus casei (Lactobacillus casei) Zhang strains obtained in step (1) into the MRS culture medium respectively, and culture them at a constant ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provide a compound lactobacillus preparation capable of obviously improving a microelement absorbing rate of saline-alkali soil peanuts, and belongs to the technical field of microorganisms. Active bacteria in the compound lactobacillus preparation are prepared from Lactobacillus plantarum P-8, Lactobacillus casei Zhang and Lactobacillus plantarum CCFM8661. The compound lactobacillus preparation has an obvious effect on growth and metabolism of the saline-alkali soil peanuts, especially can improve a utilization rate of the saline-alkali soil peanuts to soil microelements, and can improve quality of the saline-alkali soil peanuts when being applied in a peanut planting process.

Description

technical field [0001] The invention relates to a lactic acid bacteria compound preparation composed of Lactobacillus plantarum P-8, Lactobacillus casei Zhang and Lactobacillus plantarum CCFM8661 and their metabolites, especially a kind of lactic acid bacteria compound preparation which can significantly improve the trace amount of peanuts in saline-alkali soil. The invention relates to a compound preparation of lactic acid bacteria with an element absorption rate, which belongs to the technical field of microbes. Background technique [0002] Trace elements are some elements that are necessary for plants but are required in a small amount. When these elements are lacking in the soil or cannot be used by plants, the plants will not grow well. The Nanjing Soil Research Institute of the Chinese Academy of Sciences conducted a survey on the content of trace elements zinc, boron, manganese, molybdenum, copper, and iron in the soil of the whole country. The results showed that th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05G3/80A01G7/06C12R1/25C12R1/245
CPCA01G7/06C05F11/00C05G3/80C12N1/20C05F11/08
Inventor 邹晓霞赵金山张晓军刘华伟李海梅王建林王铭伦王月福
Owner 金华银河生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap