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Mycorrhizal seedling raising method of paphiopedilum micranthum

A technology of P. sclerophyllum and mycorrhizalization, applied in the biological field, can solve problems such as susceptibility to diseases and insect pests, slow growth of seedlings, poor stress resistance of tissue culture seedlings, etc.

Active Publication Date: 2017-09-05
贵州省林业科学研究院
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Tissue culture technology has become a conventional method for the production of seedlings in the orchid industry, but there are many problems to be solved when this technology is applied to the cultivation of orchids: slow growth of seedlings, poor stress resistance of tissue culture seedlings, and susceptibility to pests and diseases

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Embodiment Construction

[0025] The present invention is described further below:

[0026] The method for mycorrhizal seedling cultivation of Paphiopediphyllum sclerophyllum, the process may further comprise the steps:

[0027] a. Isolation and cultivation of mycorrhizal fungus of P. The germinated mycelium mass was taken out, and the germinated bacteria were purified and cultivated on the PDA medium for secondary purification, and finally the strains were preserved in test tubes; the two bacterial strains selected and cultivated for the cultivation of P. The basic characteristics of a kind of hyphae are: the septum is a barrel hole diaphragm, the barrel hole is covered with a pad without perforation, arc-shaped, and the mycelium cells are binucleated. The colony on BDPDA medium is light brown, smooth, uniform and thin, without aerial hyphae, growing on the medium, with concentric rings, neat edges, and fragrance. Nearly right-angled branches, 2.15-5.03μm in diameter, 17.35-119.02μm in cell length, ...

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Abstract

The invention provides a mycorrhizal seedling raising method of paphiopedilum micranthum and relates to the field of biotechnology. The method comprises the following steps: a, separating and culturing paphiopedilum micranthum mycorrhiza fungi; b, carrying out a fungus-seedling co-culturing; c, preparing a seedling cultivation medium; and d, transplanting seedlings. The invention aims to provide a method capable of taking paphiopedilum micranthum as a research material, separating fungi of mycorrhizas growing in the root of the paphiopedilum micranthum, preparing a culture medium, preparing fungus liquid, improving transplanting survival rate of tissue culture seedlings by using a mycorrhizal technology, enhancing resistance of the seedlings and shortening the growth cycle. The method is simple, is high in operability and obvious in seedling raising effect, and is capable of achieving industrial seedling raising of paphiopedilum micranthum seedlings and simultaneously treating the grown-up seedlings, the raw materials of the method is easy and convenient to obtain; the seedling raising cost can be reduced, so that higher economic benefit is achieved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a mycorrhizal symbiotic seedling raising method adopted in the seedling raising process of Sclerophyllum. Background technique [0002] In the mid-1980s, the Paphiopedilum native to my country ( Paphiopedilum ) Subgenus Broadlobe (Subgen . Brachypetalum ) of Paphiopediphyllum ( Paphiopedilum micranthum ), its flowers are beautiful and beautiful, and have very high biological research and ornamental value, which has aroused great interest of Paphiopedilum experts all over the world. Guizhou's special geographical environment makes its vegetation types rich and diverse, which provides favorable conditions for the growth of P. sclerophyllum. It is mainly distributed in the northeast to southwest of Guizhou. Due to over-collection, rampant smuggling out of the country, and habitat destruction, the number of wild P. sclerophyllum has decreased sharply in the past decade, and its dis...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01G7/06A01G9/10A01H4/00C12N1/20C12N1/02C12R1/01
CPCA01G7/06A01H4/00C12N1/02C12N1/20
Inventor 田凡王莲辉廖小锋颜凤霞姜运力罗在柒杨林霍达陈菊艳
Owner 贵州省林业科学研究院
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