A visual microbial biochemical identification kit and identification method thereof

Abstract

The invention discloses a visible microorganism biochemical identification reagent box and an identification method for same. The identification reagent box comprises a box body, in which a reaction trough and a sample loading trough connected thereto are disposed, the reaction trough arranged inclinedly. The reaction trough and the sample loading trough are respectively separated into a plurality of branch reaction troughs and branch sample loading troughs through separation plates, wherein in each branch reaction trough, a biochemical culture medium is immobilized in a manner of vacuum drying; a slope, which is used for guiding a sample solution to the reaction trough, is arranged in the sample loading trough. The reagent box achieves one-time sample loading, wherein the sample solution is distributed by means of flowability of a water solution, so that a complex process of loading a sample in every step is avoided and pollution is reduced. The reaction trough is arranged inclinedly, so that a result is easier to record when the reagent box is placed on a colorimetric card, and problems of error-recording and mis-recording are avoided. The biochemical culture mediums in the reagent box are based on sugar fermentation reaction principle and have high accuracy. The vacuum drying technology is used for drying the culture mediums and immobilizing the culture mediums on the bottom of the reaction trough, thereby forming a layer of thin film, so that the reagent is stable and is easy to preserve and transport.

Description

technical field [0001] The invention belongs to the field of microorganism detection, and in particular relates to a visualized microorganism biochemical identification kit and an identification method thereof. Background technique [0002] Food safety has become a serious problem facing our country. Food safety problems have been exposed frequently, especially in recent years, the poisoning incidents caused by food-borne pathogens have increased day by day, which poses a serious threat to people's life and health. Improving the ability to monitor food-contaminating microorganisms is of great significance to maintaining social stability in our country. [0003] In terms of microbial detection, the techniques that are often used are morphological observation, staining and biochemical characteristic reactions, molecular biology and other methods. These methods have their own advantages and disadvantages. For example, certain false positives may occur in molecular biology meth...

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Problems solved by technology

[0006] (1) There is a problem of damage, leakage and pollution during transportation, and because it is a medium in liquid form, there are disadvantages such as not easy to store, not long in validity, and inconvenient to transport;

[0007] (2) When adding samples, each vial or ampoule needs to be opened and added. The amount of addition is 1ml per well, and the amount of sample added is relatively large. During the process of adding samples, the residual liquid on the tip is easy to drip, and the operation is cumbersome, time-consuming and laborious;

[0008] (3) The substrate (biochemical medium) of the reaction is usually a microbial enzyme system reaction, and the accuracy is not as high as that of classic reactions such as sugar fermentation

Therefore, if there is a certain distance between the colorimetric card and the reaction tube, it is easy to write the result of hole A to the biochemical result of B, especially when a large number of tests are performed, it is more likely to make mistakes and cause errors in the results

[0011] (2) Use agar, or gelatin, xanthan gum, etc. to assist in fixing on the bottom of the reaction tube, drying and fixing is not firm, and it is easy to fall off, which will affect the judgment of the result

[0012] (3) The substrate (biochemical medium) of the reaction is usually a microbial enzyme system reaction, and the accuracy is not as high as that of classic reactions such as sugar fermentation

In addition, the microbial enzyme system reaction medium needs to be stored at 2-4°C, and the conditions are harsh. If the temperature deviates during transportation, it will have a greater impact on the activity of the enzyme, or lead to the failure of the enzyme substrate molecule, affecting the product. usage of

[0013] At present, domestic qualified laboratories have introduced foreign semi-automatic or fully automatic microbial numerical classification and identification systems, but the cost is high, and after-sales needs to rely on the support of foreign product technology

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