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Marker associated with the diagnosis of malignant glioma and application thereof

A technology of malignant glioma and diagnostic markers, which is applied in the direction of disease diagnosis, biomaterial analysis, biochemical equipment and methods, etc., and can solve problems such as unclear genetic characteristics

Inactive Publication Date: 2017-08-18
SHANGHAI TENTH PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Finally, the neural subtype, whose genetic characteristics are not yet clear

Method used

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  • Marker associated with the diagnosis of malignant glioma and application thereof
  • Marker associated with the diagnosis of malignant glioma and application thereof
  • Marker associated with the diagnosis of malignant glioma and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The expression of TNC gene can be detected by various techniques known in the art.

Embodiment 2

[0047] The expression product of the TNC gene (eg, Tenascin C protein) is detected by various techniques known in the art.

Embodiment 3

[0049] 1. Materials and methods

[0050] 1.1 Patient tissue samples

[0051] We collected fresh tissue samples from 8 pairs of surgically resected glioblastoma (GBM) and paired normal brain tissues according to the latest WHO classification; the samples were all from Ganzhou People's Hospital. All patients signed informed consent, and this operation was approved by the Ethics Committee of Ganzhou People's Hospital.

[0052] 1.2 Reagent preparation

[0053] UA buffer formula: Urea 48.048g, Tris 1.2114g, add 100ml water, adjust pH=8.5

[0054] IAA: 0.005M IAA dissolved in UA (13.875mg IAA dissolved in 1.5ml UA)

[0055] 200mM DTT: 30.86mg DTT dissolved in 1ml 100mM NH 4 HCO 3 middle

[0056] ABC / Ammonium bicarbonate: 0.05M ABC dissolved in H 2 O (395.3mg dissolved in 100ml ddH 2 O)

[0057] 50mM NH 4 HCO 3 : 50ul 1M NH 4 HCO 3 (79.06mg dissolved in 1ml ddH 2 O) add 950ul ddH 2 o

[0058] SDT lysate formula: SDS 4g, DTT 1.54g, Tris 1.21g, HCl to adjust pH=7.6

[...

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Abstract

The invention relates to a marker associated with the diagnosis of malignant glioma, and belonges to the technical field of molecular biology, specifically, the marker is a TNC gene and the expression product of the TNC gene. The invention also provides application of the marker. The marker has the advantages of carrying out a comprehensive genomics and proteomics analysis of GBM, and by gene expression analysis and LC-MS / MS protein mass spectrometry analysis based on gene chips and RNA sequencing, finding that the TNC gene is up-regulated at the mRNA level and protein level. Survival analysis of TNC shows that the overexpression of TNC is related to the poor prognosis of GBM patients, indicating that TNC can be used as a GBM biomarker and a potential therapeutic target.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a marker related to the diagnosis of malignant glioma and its application. Background technique [0002] The initial designation of GBM as glioblastoma multiforme was an attempt to describe the striking differences among different cells within the tumor, which also reflected molecular heterogeneity. Long before genomic technologies were able to define the molecular characteristics of GBM, a simple clinical phenomenon reshaped the concept of GBM as a single disease. Clinically, most glioblastomas (approximately 90%) are primary, and only a few progress from low-grade gliomas (secondary GBM). Primary and secondary GBMs show completely different molecular features, for example, epidermal growth factor receptor (EGFR) and phosphatase tensin homologue (PTEN) are often abnormal in primary GBMs, while TP53 is abnormal in secondary GBMs. Abnormal in episodic GBMS. Although s...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N33/574
CPCC12Q1/6886C12Q2600/118C12Q2600/158G01N33/57407G01N33/57484G01N2800/52
Inventor 符达马雨水吕中伟卢改霞
Owner SHANGHAI TENTH PEOPLES HOSPITAL
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