SNP marker location for rice bacterial leaf streak resistant gene BLS2 and application of SNP marker location
A kind of thin stripe disease and gene technology, applied in the field of molecular biology, can solve the problems of less research on molecular genetic mechanism and in-depth research on molecular basis, and achieve the effect of improving selection efficiency
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[0037] 1. Construction of Multiparent Advanced Generation Inter-Cross (MAGIC)
[0038] Before constructing the population, the resistance identification of the three parents was carried out, and the results were as follows: O. Susceptible 998R hybridization, F1 self-crossing for 10 consecutive generations, obtained Multiparent Advanced Generation Inter-Cross (MAGIC) S10, and carried out resistance identification on the obtained multi-parent Advanced Generation Inter-Cross (MAGIC) population, Obtain 30 extremely disease-resistant individual plants and mix them to construct a disease-resistant pool (N915) for sequencing; obtain 30 extremely susceptible individual plants and mix them to construct a disease-resistant pool (N908) for sequencing.
[0039] 2. Identification of resistance
[0040] At the tillering stage of rice, the representative strain JZ28 (type Ⅲ) of dominant pathogenic bacterial leaf spot widely distributed in Guangxi was used for inoculation. After the strain ...
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