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Preparation method of efficient nematicidal combination fungicide

A combined fungicide and nematicide technology, applied in the directions of nematicides, botanical equipment and methods, microorganism-based methods, etc., can solve problems such as difficult treatment of prolifera, environmental pollution, and no treatment methods, and achieve good killing effect. Nematode effect, substantial advancement, effect of improving fertility

Inactive Publication Date: 2017-08-18
BEIJING ZNFY GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The salvaged Enteromorpha is not easy to treat, and there is no suitable treatment method at present. Letting Enteromorpha pile up will bring another environmental pollution problem

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A preparation method for a highly efficient nematode-killing combined bacterial agent, comprising:

[0025] Step (1), preparation of Metarhizum anisopliae suspension; Metarhizum anisopliae (Metarhizum anisoplia, preservation number is CGMCC No.8385, purchased through commercial channels) strains were inoculated on PDA slant medium at 24°C and cultivated for 5 day, the formula of the PDA slant medium includes, in parts by weight, 5 parts of potato flour, 1 part of sucrose, 5 parts of corn flour, MgSO 4 ·7H 2 O0.1 copies, and K 2 HPO 4 0.2 parts, washed with deionized water after the cultivation, the washed liquid is the suspension of Metarhizium anisopliae;

[0026] Prepare photosynthetic bacterium acidophilus acidophilus PSB-1 ​​suspension (Rhodoblastus acidophilus, preservation number CCTCC NO.M 206124, purchased through commercial channels), put photosynthetic bacteria acidophilus PSB-1 ​​strain in liquid Cultivate and ferment in the culture medium, cultivate 5 d...

Embodiment 2

[0033] A preparation method for a highly efficient nematode-killing combined bacterial agent, comprising:

[0034] Step (1), preparation of Metarhizium anisopliae suspension; Metarhizium anisopliae strains were inoculated at 24°C on a PDA slant medium and cultivated for 10 days, and the formula of the PDA slant medium included, in parts by weight, potatoes 10 parts of flour, 5 parts of sucrose, 20 parts of corn flour, MgSO 4 ·7H 2 O 5 parts, and K 2 HPO 4 3 parts, washed with deionized water after the cultivation, and the washed liquid was the suspension of Metarhizium anisopliae;

[0035] Prepare the photosynthetic bacterium acidophilus P. paella PSB-1 ​​suspension, put the photosynthetic bacterium acidophilus P. paella PSB-1 ​​strain into the liquid medium for cultivation and fermentation, and cultivate it under 4000LX light irradiation for 7 days; wherein, the liquid The medium includes, in parts by weight, 10 parts of sucrose, 2 parts of potassium acetate, MgSO 4 ·7H...

Embodiment 3

[0042] A preparation method for a highly efficient nematode-killing combined bacterial agent, comprising:

[0043] Step (1), preparation of Metarhizium anisopliae suspension; Metarhizium anisopliae strains were inoculated on PDA slant medium at 24°C and cultivated for 6 days, and the formula of this PDA slant medium included, in parts by weight, potato 6 parts of flour, 5 parts of sucrose, 10 parts of corn flour, MgSO 4 ·7H 2 O 0.3 parts, and K 2 HPO 4 0.3 parts, washed with deionized water after the cultivation, the washed liquid is the suspension of Metarhizium anisopliae;

[0044] Prepare the photosynthetic bacterium acidophilus paella rhododendron PSB-1 ​​suspension, place the photosynthetic bacterium acidophilus paella rhododendron PSB-1 ​​strain in a liquid medium for cultivation and fermentation, and cultivate it under natural light for 30 days; wherein, the liquid The medium includes, in parts by weight, 10 parts of sucrose, 2 parts of potassium acetate, MgSO 4 ·...

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PUM

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Abstract

The invention discloses a preparation method of efficient nematicidal combination fungicide. The method comprises the steps that a metarhizium anisopliae suspension is prepared; a photosynthetic bacterium rhodoblastus acidophilus PSB-1 suspension is prepared; a krusei candida mycoderma bacterium TY9 suspension is prepared; a culture mixed solution is prepared; enteromorpha is prepared and solid fermentation is performed; according to the long-acting biological nematicidal combination fungicide, the content of metarhizium anisopliae is 3*10<6> cfu.<-1> or above, the content of the photosynthetic bacterium rhodoblastus acidophilus is 2*10<7> cfu.<-1> or above, and the content of the krusei candida mycoderma bacterium is 1*10<7> cfu.<-1> or above. By adopting the three types of bacteria, the enteromorpha is adopted as a fermentation raw material to be fermented, the fermentation product has the good nematicidal effect, fertilizer with abundant nitrogen phosphorus and potassium and microelements can be provided, the control rate is 85-100%, and the yield of crops is increased by 5-15%.

Description

technical field [0001] The invention relates to the field of preparation of agricultural nematocide agents, in particular to a method for preparing a highly efficient nematicide-killing combined bacterial agent. Background technique [0002] Root-knot nematode is a widely distributed and serious plant parasitic nematode, which causes great economic losses to agricultural and forestry production. Root-knot nematodes can directly damage the epidermal cells of the host to cause mechanical damage to the crops, and can also stab the host through the stylet, secrete toxic substances, form nodular root knots, and affect the growth of crops. Almost all tree species are susceptible to these pests, which affect the roots of host plants but can also damage above-ground parts, including stems, leaves and flowers. [0003] Methods of controlling nematodes, most previous attempts at nematode control involving the application of chemical nematode repellents and / or microorganisms such as c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C12N1/14A01N63/04A01N63/00A01N63/02A01P5/00C12R1/645C12R1/01C12R1/72
CPCA01N63/00A01N63/10A01N63/30C12N1/14C12N1/16C12N1/20A01N2300/00
Inventor 韩文贺屈庆涛屈智闻
Owner BEIJING ZNFY GRP CO LTD
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