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Erysipelothrix rhusiopathiae antigen protein sbp and application

A technology of Erysipelothrix rhusiopathiae and antigenic protein, applied in the direction of bacterial antigenic components, applications, bacterial peptides, etc., can solve the problems of high cost, large protein molecular weight, and low conservation of SpaAN end, and achieve good protection effect and biological safety high effect

Active Publication Date: 2017-08-11
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the molecular weight of the protein is relatively large, and it is difficult to obtain the full-length protein, and the cost is relatively high
Research on the protection of the N-terminal truncated protein found that it can also provide good protection for piglets, but the N-terminus of SpaA is not highly conserved. Whether the truncated protein can protect pigs against different Erysipelothrix infections currently endemic in China is uncertain

Method used

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  • Erysipelothrix rhusiopathiae antigen protein sbp and application
  • Erysipelothrix rhusiopathiae antigen protein sbp and application
  • Erysipelothrix rhusiopathiae antigen protein sbp and application

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Effect test

Embodiment 1

[0025] Preparation of Erysipelothrix rhusiopathiae antigenic protein (the present invention or claiming sbp protein):

[0026] The embodiment of the present invention takes prokaryotic expression as an example to illustrate the expression of the protein:

[0027] Construction of Escherichia coli BL21 / pET-28a-sbp, expression and purification of target protein

[0028] 1) Construction of expression vector

[0029] The sbp gene (sequence shown in SEQ ID NO.1) was inserted between the BamHI and XhoI restriction sites of pET-28a(+) according to conventional methods to construct the recombinant vector pET-28a-sbp. Transform into DH5a competent strains, pick positive clones to expand and culture, extract plasmids, use BamHI+XhoI to double-enzyme-digest the plasmids, and the foreign fragments of the expected size after digestion are correct recombinant plasmids. The results of double enzyme digestion identification of the recombinant plasmid were as follows: figure 1 As shown, the ...

Embodiment 2

[0034] Example 2: Subunit vaccine components and routine testing

[0035] Antigen component: sbp protein solution (expression, identification, purification and quantification of protein are as described in Example 1).

[0036] Dosage form: water-in-oil (W / O)

[0037] Adjuvant composition: white mineral oil (Marcol52)

[0038] Tween 80 (CRILLET4)

[0039] Span 80 (CRILL4)

[0040] Oil phase: heat white mineral oil to transparent and prepare with Span 80 at a volume ratio of 94:6.

[0041] Water phase: the sbp protein solution is composed of Tween 80, and Tween 80 accounts for 0.4% (volume ratio) of the water phase.

[0042] Vaccine emulsification: sbp protein is added to Tween 80 and mixed to form a water phase, and the water phase and oil phase are mixed and emulsified at a volume of 1:1.5. The final concentration of the antigenic protein sbp is 1mg / ml, which is used for the following experiments. The sbp protein vaccine provided by the present invention is also called sb...

Embodiment 3

[0047]Application of a kind of Erysipelothrix rhusiopathiae antigenic protein sbp in the preparation of porcine erysipelas subunit vaccine:

[0048] 1) Experimental scheme

[0049] Experimental animals: 30-day-old piglets (negative for Erysipelothrix rhusiopathiae antibody), purchased from Wuhan Tianzhong Qingcaohu Breeding Co., Ltd.

[0050] Attacking strain: Erysipelothrix rhusiopathiae SE38 strain (hereinafter referred to as SE38, Li Jingtao, Analysis of drug resistance of Erysipelothrix isolates in parts of Hubei, Breeding and Feed [J]. 2015.2, 11-14).

[0051] Experimental grouping: 30-day-old piglets were divided into 3 groups: immunization group 1 (sbp), immunization group 2 (sbp), adjuvant control group (adjuvant components described in Example 2 mixed with Tris-HCl), 6 pigs in each group . The immunization group 1 and the adjuvant control group were used for challenge, and the immunization group 2 was used to detect the antibody level, and the detection continued fo...

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Abstract

The invention discloses erysipelothrix rhusiopathiae antigen protein sbp and an application. The amino acid sequence of the protein sbp is shown in SEQ ID NO.2. The protein is mixed with an adjuvant in proportion after aseptic treatment, piglets are immunized intramuscularly, it is found that the protein can effectively increase the survival rate of the piglets after challenge and increase the recovery speed of the piglets, and safety tests prove that immunized piglets have no adverse effects; meanwhile, the corresponding nucleotide sequence of the protein is compared with the sequences of 31 erysipelothrix rhusiopathiae, and the homology is found to be higher than or equal to 98%. Therefore, the antigen protein is highly conservative in different clinical isolates and can protect pig groups against attack of the current epidemic strains.

Description

technical field [0001] The invention relates to the field of livestock genetic engineering vaccines, in particular to an Erysipelothrix rhusiopathiae antigenic protein SBP and its application. [0002] technical background [0003] Erysipelothrix rhusiopathiae infects pigs and causes swine erysipelas, which is one of the three major infectious diseases of pigs in the 1990s. Later, due to the use of commercial vaccines, porcine erysipelas was gradually effectively controlled. However, since 2010, swine erysipelas has re-emerged in some provinces and cities in my country. In 2012, swine erysipelas cases have gradually increased. By 2013, swine erysipelas had become widespread in many provinces such as Hunan, Hubei, Jiangxi, Guangxi, Sichuan, Guangdong, and Zhejiang in my country, causing huge economic losses to pig farms. [0004] Penicillin has been an effective drug for the treatment of swine erysipelas. However, in 2011, He Shicheng and others from the Hunan Animal Disea...

Claims

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Application Information

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IPC IPC(8): C07K14/195C12N15/31A61K39/02A61P31/04C12R1/01
CPCA61K39/0208A61K2039/552C07K14/195
Inventor 金梅林王雅李敬涛吴超孙小美
Owner HUAZHONG AGRI UNIV
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