Total internal reflection fluorescence microscopic imaging device of dual variable-diameter ellipsoidal reflector

Abstract

The invention relates to a total internal reflection fluorescence microscopic imaging device of a dual variable-diameter ellipsoidal reflector and belongs to the field of optical microscopic illumination. The device uses annular light modulated by a diaphragm and a shading film, generates a large-vertex-angle hollow-cone light beam focused at the first focal point of the ellipsoidal reflector after the annular light is reflected by the ellipsoidal reflector, thereby achieving a total internal reflection fluorescence imaging phenomenon. The device can change the vertex angle of the hollow cone by moving the shading film and adjusting the diaphragm so as to finally change the excitation depth of an evanescent field and to expand the axial image of a fluorescence image. The shading film and the diaphragm can be adjusted in parallel so as to enhance the applicability of the instrument. The annular illumination light from 0 to 360 degrees provided by the device can achieve shadowless illumination, thereby reducing the interference fringes in the image, increasing the contrast of the image, and improving image quality.

Description

technical field [0001] The double variable-diameter ellipsoid reflector total internal reflection fluorescent microscopic imaging device of the present invention belongs to the field of optical microscopic imaging, and has wide applications in the research fields of life science, physical chemistry, material science and the like. Background technique [0002] With the discovery of new mechanisms in the field of life sciences and the synthesis of new substances in the field of materials science, optical microscopes have developed rapidly, and some high-tech technologies have continued to make breakthroughs. A large number of facts in life sciences prove that the dynamic characteristics of cells originate from the aggregation and interaction of individual protein molecules. To this end, it is necessary to vigorously develop super-resolution imaging technology, so as to detect the details of various life activities of cells at the molecular scale, and further reveal the process...

AI Technical Summary

Problems solved by technology

In this method, although the objective lens with large numerical aperture (NA) can provide large-angle illumination, the adjustment range of the incident angle is still limited, so the excitation depth is also limited within a certain range.

[0004] The semi-cylindrical lens can change the incident angle to control the excitation depth, but this method of adjusting the excitation depth has a high degree of freedom and requires the use of more precision optical devices, which increases the difficulty of adjusting the optical path

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