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Staining method convenient for observing digestive systems of predatory mites and prey flour mites

A technology of digestive system and dyeing method, applied in the direction of animal husbandry, etc., can solve the problem of not observing the digestive system of predatory mites and their prey meal mites, etc., and achieve the effect of simple operation

Inactive Publication Date: 2017-07-14
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
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Problems solved by technology

[0004] However, so far, there is no relevant method to observe the digestive system of predator mites and their prey acarina mites in China.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0008] Example The dyeing method of tyrophagous mite and predatory mite

[0009] Under the conditions of temperature 22°C-27°C and humidity 75%-85%, mix Sudan red into the feed wheat bran of tyrophagous mite at a ratio of 1:2, mix well, and feed putty tyrophagous mite in a feeding box. mites. After 12-24 hours, the abdomen of the tyrophagous mite was stained red. The digestive system of the whole tyrophagous mite can be observed under the dissecting microscope. Put several heads of predatory mites that have been starved for 24 hours and can feed on tyrophagous mite into the breeding box. After 12-24 hours, it can be observed under the dissecting microscope that the predatory mites are red all over, and the color of the digestive tract is darker.

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Abstract

The invention relates to a staining method convenient for observing the digestive systems of predatory mites and prey flour mites. The staining method is characterized in that sodium fluorescein or Sudan red is mixed with a flour mite feed at the mass ratio of (1:1)-(1:10), the flour mites are fed with the feed mixed with sodium fluorescein or Sudan red, the flour mites of which the digestive systems can be conveniently observed can be obtained within 12-48 h, the obtained flour mites are continuously used for raising the predatory mites, and the predatory mites of which the digestive systems can be conveniently observed can be obtained within 12-48 h. The staining method is simple to operate, and can provide technical references for observing the digestive tracts and the whole digestive systems of the predatory mites and alternative preys thereof under a fluorescence microscope or a stereo-anatomical lens.

Description

technical field [0001] The invention relates to a dyeing method for conveniently observing the digestive system of predatory mites and their preys, which belongs to the field of biological control of modern agricultural pests. Background technique [0002] At present, domestic research on mites is mainly limited to classification, breeding and application. There is little research on the microstructure of mites, especially the mechanism of the digestive system. [0003] The mite digestive system is an important structure for the study of mites eating and digesting food. It is of great significance to observe the direction of food in the mite body after the mite eats the food. . [0004] However, so far, there is no relevant method in China on how to observe the digestive system of predator mites and their prey Acarina mites. Contents of the invention [0005] The purpose of the present invention is to provide a dyeing method that is convenient for observing the digestive...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 姜晓环徐学农贾冰红刘珊王恩东吕佳乐岳瑾乔岩董杰周长青张正伟王伟青庞博张乐
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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