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Near infrared spectrum measuring method for content of urea nitrogen and content of creatinine in serum

A technology of near-infrared spectroscopy and urea nitrogen creatinine is applied in the field of near-infrared spectroscopy measurement of blood urea nitrogen and creatinine content in serum, which can solve the problems of result interference, negative deviation, interference, etc., achieve short detection time, avoid human error and cost of operation low effect

Inactive Publication Date: 2017-07-07
SHANGHAI UNIV OF MEDICINE & HEALTH SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, although the Jaffe method has relatively cheap reagents, good repeatability and accuracy, it is susceptible to interference from other pseudo-creatinine substances in the serum, especially when the serum bilirubin value ≥ 165.5 μmol / L, negative deviations begin to appear
In addition, drugs such as cephalosporins, vitamin C and dopamine also greatly interfere with the results

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] This embodiment provides a near-infrared spectroscopy method for the determination of urea nitrogen and creatinine content in serum:

[0025] Step 1. Use a biochemical analyzer or detection kit to determine the serum urea nitrogen content and creatinine content, and prepare several serum calibration solutions with different urea nitrogen content and creatinine content.

[0026] Among them, the quantity of serum calibration solution needs to meet the requirements of establishing a model. Preferably, the number of serum calibration solutions is at least 30.

[0027] The range of urea nitrogen content of several serum calibration solutions is 2-7mmol / L, and the range of creatinine content is 20-200umol / L.

[0028] Step 2: Use a near-infrared spectrometer to collect the spectrum of each serum calibration solution.

[0029] Step 3: Establish a urea nitrogen identification model and a creatinine identification model based on the near-infrared spectrum of the serum calibration solution...

Embodiment 2

[0035] This embodiment provides a near-infrared spectroscopy method for the determination of urea nitrogen and creatinine content in serum, which is basically the same as the method in Example 1, except that: Step one uses the urea nitrogen and creatinine dual quantitative detection method to determine the serum urea nitrogen content and Creatinine content to prepare several serum calibration solutions with different urea nitrogen content and creatinine content.

[0036] The specific steps of the two-item quantitative detection method for urea nitrogen and creatinine are:

[0037] Step I: Take out two identical serums from the serum to be tested for urea nitrogen and creatinine content.

[0038] Step II: Add reagent a and reagent b to two serums at the same time, mix well, and incubate at 37°C for 5 min.

[0039] In this embodiment, the temperature of the warm bath can also be 36°C, correspondingly, the warm bath time is 5 minutes; or, the temperature of the warm bath can also be 38°C...

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Abstract

The invention discloses a near infrared spectrum measuring method for the content of urea nitrogen and the content of creatinine in serum. The method includes the steps that 1, multiple kinds of serum scaling liquid with different urea nitrogen contents and different creatinine contents are prepared; 2, a near infrared spectrograph is used for carrying out spectrum acquisition on each kind of serum scaling liquid; 3, a urea nitrogen identification model and a creatinine identification model are established according to the near infrared spectrums and the contents of urea nitrogen and creatinine in the serum scaling liquid; 4, the near infrared spectrograph is used for carrying out spectrum acquisition on a to-be-detected serum sample, a spectrum of the serum sample is compared with the urea nitrogen identification model and the creatinine identification model, and the content of urea nitrogen and the content of creatinine in the serum sample are obtained. The method has the advantages of being short in detection time, high in detection efficiency, high in detection accuracy and the like.

Description

Technical field [0001] The invention belongs to the field of blood testing, and relates to a method for determining the content of urea nitrogen and creatinine in serum, and in particular to a method for determining the content of urea nitrogen and creatinine in serum by near infrared spectroscopy. Background technique [0002] Urea is the main end product of human protein metabolism. Creatinine is the product of muscle metabolism in the human body. Urea and creatinine are mainly filtered out of the body by glomerular filtration, so they can be used for the diagnosis and screening of glomerular filtration function. Indicator, when the glomerular filtration function decreases, blood creatinine and urea nitrogen increase due to retention. [0003] At present, the enzymatic method or alkaline picric acid method (Jaffe method) is commonly used to determine blood and urine creatinine in clinical laboratories. Among them, although the reagents of the Jaffe method are cheaper, repeatabil...

Claims

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Application Information

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IPC IPC(8): G01N21/359G01N21/3577
Inventor 黄钢王海波杨培强徐军胡兆燕
Owner SHANGHAI UNIV OF MEDICINE & HEALTH SCI
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