Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing prednisolone through bio-fermentation in one step

A technology of biological fermentation and prednisolone, which is applied in the field of preparation of prednisolone, can solve the problems such as no process route and adjustment.

Inactive Publication Date: 2017-06-27
TIANJIN JINYAO GRP
View PDF9 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, researchers have made more efforts to innovate and improve the single-step process. However, due to traditional empirical thinking, the adjustment of the process route has not yet been carried out.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing prednisolone through bio-fermentation in one step
  • Method for preparing prednisolone through bio-fermentation in one step
  • Method for preparing prednisolone through bio-fermentation in one step

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Arthrobacter Simple ATCC 21032 is cultured in slant medium, primary culture and secondary culture in sequence, and the culture temperature is 29-32°C, and hydrocortisone acetate dissolved in an organic solvent is put into the fermentation medium In the 5L fermenter, the feed concentration is shown in the table below, and the reaction temperature is 31±1°C. The reaction time is shown in the table below. After the reaction is completed, the temperature is raised to 70°C to terminate the reaction, the fermentation broth is extracted with ethyl acetate, the organic phase is concentrated, and the conversion rate of prednisolone is measured.

[0051]

Embodiment 2

[0053] The specific preparation method culture medium and feeding concentration are the same as in Example 1, except that Tween 80 is added to the 5L fermentation tank of the fermentation medium before cortisone acetate is put into use.

[0054]

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for directly generating prednisolone through bio-fermentation in one step by using cortisone acetate as a raw material and Arthrobacter Simple ATCC 21032.

Description

[0001] Technical field: [0002] The invention relates to a preparation method of prednisolone, which is to prepare prednisolone from hydrocortisone acetate in one step by carrying out 1, 2-position dehydrogenation and 21 acetate hydrolysis reactions through biological fermentation. [0003] Background technique: [0004] Prednisolone, a common corticosteroid, can be used not only as a corticosteroid treatment drug, but also as a raw material for other corticosteroid drugs. It has wide application value and is very important. Research on the preparation of this product continues at home and abroad. [0005] [0006] The commonly used starting material for domestic production of prednisolone is mold oxide derived from diene, which is first oxidized at C11, then dehydrogenated at C1 and 2, and then modified at the side chain to obtain esterification at 21. The difficulty of this process lies in the introduction of C11-position carbonyl and C1, 2-position double bonds. Since t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P33/02C12P33/00C12R1/06
CPCC12P33/005C12P33/02
Inventor 王福军张杰王延年
Owner TIANJIN JINYAO GRP
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com