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Preparation method of targeted exosome carrying anti-tumor protein and application thereof

An anti-tumor, protein-targeting technology, applied in anti-tumor drugs, polypeptides containing localization/targeting motifs, peptide/protein components, etc. problems, to achieve the best clinical application value, low cost, and low difficulty in operation.

Inactive Publication Date: 2017-06-27
SHANGQIU MEDICAL COLLEGE
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Problems solved by technology

[0008] The purpose of the present invention is to provide a preparation method and application of an anti-tumor protein-targeted exosome, aiming to solve the problem that the preparation process of the current exosome is relatively complicated, the operation process is cumbersome, the work efficiency is low, and the prepared exosome cannot be corrected. The problem of verifying the effect

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  • Preparation method of targeted exosome carrying anti-tumor protein and application thereof

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[0027] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0028] The application principle of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0029] Such as figure 1 As shown, the preparation method of the anti-tumor protein-targeted exosome provided by the embodiment of the present invention includes the following steps:

[0030]S101: Take 1 μl of the sample product and add it to 100 μl exosome membrane protein competent cells, and ice-bath for 30 minutes. Then heat shock at 42°C for 60s, immediately ice-bath for 2min; add 500μl non-resistant LB liquid medium, incubate at 200rpm at 37°C for 1h, take the bacterial solution...

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Abstract

The invention discloses a preparation method of a targeted exosome carrying an anti-tumor protein and application thereof. The exosome is extracted from a sample by adopting the preparation method of the targeted exosome carrying the anti-tumor protein. The exosome is separated and purified, and exosome capsule particles are extracted. The growth of tumors can be efficiently inhibited, a potential ideal treatment method is provided for malignant tumor treatment, the exosome is extracted by utilizing a separation and purification method, separation can be conveniently, efficiently and standardly performed, the process is simple, and operation is convenient. The working efficiency is improved.

Description

technical field [0001] The invention relates to the technical field of molecular cell biology, in particular to a preparation method and application of an anti-tumor protein targeting exosome. Background technique [0002] In recent years, based on stem cells, especially mesenchymal stem cells (MSCs) (adipose stem cells are a type of mesenchymal stem cells, compared with other mesenchymal stem cells, adipose stem cells have the advantages of easy access to materials and no ethical restrictions) Research on skin regenerative medicine has received extensive attention. Studies have shown that MSC has a good repair effect on tissue damage. Although its mechanism has not been fully elucidated, preliminary results show that it may be related to the stimulation of damage and various cytokines in the local wound microenvironment. They It participates in the repair of damaged tissues by promoting MSC chemotaxis and inducing MSCs to differentiate into required tissues or cells, so it ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/10C12N15/85C07K19/00A61K38/17A61K47/66A61P35/00A01K67/027
CPCA01K67/027A01K2227/105A01K2267/0331A61K38/00C07K14/47C07K2319/01
Inventor 宋凌杨晓莉王钟辉李娜王春艳
Owner SHANGQIU MEDICAL COLLEGE
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