Preparation method of high-efficiency fermentation liquid culture medium

Abstract

The invention relates to a preparation method of a high-efficiency fermentation liquid culture medium. The method comprises the following steps: mixing hydrochloric acid and trimethylamine at normal temperature to synthesize trimethylamine hydrochloride, introducing epoxyethane into the trimethylamine hydrochloride to generate a condensation solution, transferring the condensation solution into a vacuum concentration evaporator, and evaporating to obtain a high-effective-content choline chloride water solution; and injecting water into a reaction kettle, adding chloroacetic acid and anhydrous sodium carbonate, quantitatively adding trimethylamine, heating the mixture until all salts completely settle, carrying out centrifugal drying to remove the salts, injecting the mother solution obtained by centrifugal drying into a concentration kettle, re-concentrating to obtain betaine, and finally, mixing the choline chloride water solution and betaine. The production method is simple to operate and suitable for large-scale production, and has the advantages of low production cost and the like. The fermentation liquid culture medium produced by the production method is mainly composed of the choline chloride, and the betaine is used as a methyl donor in the culture medium, thereby promoting the strain component optimization, inducing the single direction fermentation of the strain, reducing the byproducts, and enhancing the titer quantity of the product.

Description

technical field [0001] The invention belongs to the technical field of biochemical industry, and in particular relates to a preparation method of a high-efficiency fermentation broth medium. Background technique [0002] Fermentation medium is used for the growth, reproduction and synthesis of bacteria. It not only enables the seeds to grow rapidly after inoculation to reach a certain mycelial concentration, but also enables the grown bacteria to rapidly synthesize the required products. Therefore, it is required that the composition of the fermentation medium should be rich and complete. The carbon and nitrogen sources should pay attention to the mutual matching of quick-acting and late-acting nutrients. pH value; and there are also growth factors required for cell growth and elements, precursors and promoters required for product synthesis. In addition to the elements and compounds necessary for the growth of bacteria, there are also specific elements, precursors and acc...

AI Technical Summary

Problems solved by technology

The current fermented broth medium strains ferment more impurities and complex components, which affect the potency of the product