Culture medium for methanosarcina

Abstract

The invention provides a culture medium for Methanosarcina, which comprises the following ingredients in parts by weight: 8 parts of sorghum flour, 6 parts of corn flour, 2 parts of wax gourd, 1 part of Glauber's salt, and 1 part of oriental cypress leaves , 1 part of yellow ox tea, 1 part of Tripterygium wilfordii; on this basis, a specific amount of xylitol, tangerine peel or ginseng leaves can be added. On the basis of the commonly used materials in the culture medium, certain Chinese herbal medicines are added to the formula, which unexpectedly achieves outstanding cultivation effects, and is especially suitable for the cultivation of Methanosarcina, with good application prospects.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a culture medium for Methanosarcina. Background technique [0002] Typical methanosarcina are octachest-shaped, sometimes aggregated as large cysts, with a common outer wall enclosing individual spherical cells, do not produce spores, variable Gram staining. Individual spherocytes may be sensitive to detergent lysis or hypertonicity. Do not exercise. Extremely strictly anaerobic. The optimum NaCl concentration is 0.1-0.5mol / L, the optimum temperature for mesophilic bacteria is 30-40°C, and the optimum temperature for thermophilic bacteria is 50-55°C. Organic growth factors are usually not required. Energy metabolism manifested as disproportionation of methylamine or methanol to CH 4 and CO 2 and NH 3 . The strain has a certain application in the field of biotechnology, but the proliferation speed of Methanosarcina in the commonly used culture medium is relatively s...

AI Technical Summary

Problems solved by technology

[0003] The present invention aims at the technical defects of the prior art, and provides a culture medium for Methanosarcina to solve the technical problem of slow proliferation of Methanosarcina in the prior art