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Rice variety IR24 black-streaked dwarf virus resistant site and molecular marker method thereof

A technology for rice black-streaked dwarf and black-streaked dwarf disease, applied in the field of rice variety IR24 black-streaked dwarf disease-resistant loci and its molecular markers, can solve the problems of speeding up the breeding process of disease-resistant varieties, time-consuming, labor-intensive, and poor repeatability, etc. problem, to achieve the effect of accurate and credible phenotypic identification method, clear position, and accelerated breeding process

Inactive Publication Date: 2017-06-13
YIELEAD RICE IND
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Problems solved by technology

[0009] The purpose of the present invention is to provide a rice variety IR24 black-streaked dwarf resistance site and its molecular markers for the shortcomings of rice black-streaked dwarf resistance identification stability, poor repeatability, time-consuming and labor-intensive problems in conventional breeding The method, which can predict the resistance of plants to rice black-streaked dwarf disease, simplifies the selection method, and then accelerates the breeding process of disease-resistant varieties

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  • Rice variety IR24 black-streaked dwarf virus resistant site and molecular marker method thereof
  • Rice variety IR24 black-streaked dwarf virus resistant site and molecular marker method thereof

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Embodiment Construction

[0031] The methods used in the following examples are conventional methods unless otherwise specified.

[0032] 1. Antigen screening and IR24 / Asominori F2 population construction:

[0033] From 2009 to 2012, 325 rice resources were screened for resistance to black-streaked dwarf disease by multi-year multi-point method for two consecutive years. The results showed that the average incidence rate of IR24 was 6.8%, which belonged to the level of medium resistance. The rice black-streaked dwarf resistance source IR24 was used as the female parent, and the rice black-streaked dwarf high-susceptibility variety Asominori was used as the male parent, and the hybrid F1 was constructed. A F2 segregation population containing 268 individuals was obtained, and F2:3 families were obtained by selfing of F2 individuals.

[0034] 2. Acquisition of resistance phenotype parameters of F2:3 family:

[0035] For the F2:3 family, the method of combining natural identification in the field and id...

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Abstract

The invention relates to a rice variety IR24 black-streaked dwarf virus resistant site and a molecular marker method thereof. According to the rice variety IR24 black-streaked dwarf virus resistant site and the molecular marker method, the filial generation F2 population of a rice black-streaked dwarf virus resistant variety IR24 and a susceptible variety is adopted as a mapping population; the rice black-streaked dwarf virus resistance is identified by using a combined method of natural field identification and improved laodelphax striatellus antixenosis identification; linkage analysis is carried out on virus resistance identification results and SSR marker data to obtain a SSR marker, closely linked to rice black-streaked dwarf virus resistance major QTL, on the chromosome 8; the resistance of a plant to the rice black-streaked dwarf virus can be predicated by detecting banding pattern data of the primer marker on the chromosome 8. Therefore, the selecting efficiency of black-streaked dwarf virus resistant rice is greatly enhanced and the rice breeding progress is accelerated.

Description

technical field [0001] The invention relates to a rice variety IR24 anti-black-streaked dwarf locus and a molecular marking method thereof, belonging to the field of rice molecular breeding science and technology. Background technique [0002] Rice black-streaked dwarf disease is a viral disease caused by Rice black-streaked dwarf virus (RBSDV), which is mainly transmitted by the small planthopper. There are 28 species of hosts including hybrid rice, conventional rice, barley, wheat, corn, millet, sorghum, kangaroo, bluegrass, barngrass, barnyardgrass, and matang. Rice black-streaked dwarf virus infects corn and causes corn rough dwarf disease, and infects wheat to cause wheat dwarf disease. The pathogenic forms, hosts and symptoms, vector insects and disease transmission characteristics of these diseases are very similar. Rice black-streaked dwarf disease can occur at the rice seedling stage, tillering stage, and jointing stage. Due to different infection stages, the sympt...

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6895C12Q2600/156
Inventor 乔保建邓建社陶颖付锡江任代胜陶元平
Owner YIELEAD RICE IND
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