Transwell micro-fluidic chip combined with three-dimensional culture and preparation method of micro-fluidic chip

Abstract

The invention discloses a transwell micro-fluidic chip combined with three-dimensional culture and a preparation method of the micro-fluidic chip. The micro-fluidic chip is mainly composed of a top chip layer, a porous filtration membrane, an intermediate chip layer and a bottom chip layer, wherein the porous filtration membrane seals the lower surface of the top chip layer in an irreversible manner and is adhered to the upper surface of the intermediate chip through PDMS (Polydimethylsiloxane). A pit structure of a bottom culture room is used for three-dimensional culture of in-vitro cells, tissues and organs. The transwell micro-fluidic chip disclosed by the invention simultaneously has functions of a transwell chamber, a micro-fluidic chip and in-vitro three-dimensional culture, can achieve the effect of observing cell growth conditions on the bottom chip in real time and can be applied to biological studies such as drug metabolism, organ development and the like.

Description

technical field [0001] The invention relates to the field of microfluidic chip preparation, in particular to a transwell microfluidic chip combined with three-dimensional culture and a preparation method thereof. Background technique [0002] Transwell experimental technology, the traditional method is to put the commercialized Transwell chamber into the culture plate, the chamber is called the upper chamber, and the culture plate is called the lower chamber, the upper chamber is filled with the upper layer of culture fluid, the lower chamber is filled with the lower layer of culture fluid, and the upper and lower layers of culture fluid are separated by a polycarbonate membrane. We planted the cells in the upper chamber, and because the polycarbonate membrane is permeable, the components in the lower layer of the culture medium can affect the cells in the upper chamber, so that we can study the effects of the components in the lower layer of the culture medium on cell growt...

AI Technical Summary

Problems solved by technology

Ordinary cell cultures gradually lose their original traits due to the proliferation of cells in a changed environment in vitro, and the gene expression, signal transduction, and morphology of cells may be different from natural ones, which are often inconsistent with the situation in vivo, while animal experiments completely It is carried out in the body, but it becomes complicated due to the constraints of various factors in the body and the interaction between the body and the external environment, it is difficult to study a single process, and it is difficult to study the intermediate process

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