Cell counting and sorting device

Abstract

The invention provides a cell counting and sorting device, comprising a counter body seat, wherein the counter body seat is connected with a laser transmitter; a jet pipe seat, a gem hole bracket and a counter body are arranged in sequence from top to bottom on the counter body seat; an injection hole is formed in the middle of the jet pipe seat and used for injecting cells to be measured; an inner sheath fluid inlet is formed beside the injection hole; an outer sheath fluid inlet is formed at the end part of the gem hole bracket; a gem hole for cells and inner sheath fluid to pass through is formed in the center of the bottom of the gem hole bracket; the counter body is used for counting cells flowing through the gem hole; a laser light scattering lens is arranged below the gem hole; a through hole is formed in the laser light scattering lens; the outer sheath fluid inlet is inserted into the through hole; and the cells are sorted by collecting scattered signals produced when laser emitted by the laser transmitter is scattered by the cells in the laser light scattering lens. The cell counting and sorting device can simultaneously count cells and perform laser light scattering sorting on the cells, thus reducing equipment cost and improving test efficiency.

Description

technical field [0001] The invention relates to the technical field of medical instruments, in particular to a cell counting and classifying device. Background technique [0002] Routine blood examination is one of the most common laboratory tests in clinical practice. Routine blood examination items mainly include red blood cells, white blood cells, platelets, hemoglobin, and white blood cell classification. Systemic diseases, reflecting the hematopoietic function of the bone marrow, etc. [0003] White blood cells (WBC for short) are a type of cells in the blood. White blood cells are also commonly called immune cells. It can be seen under a microscope that blood cells are relatively large in size and relatively small in number. have a nucleus. Its main function is to phagocytose bacteria and defend against diseases. At present, white blood cells are detected by using five-classification technology, that is, white blood cells are divided into lymphocytes, monocytes, ...

AI Technical Summary

Problems solved by technology

However, at present, most five-classification instruments design cell counting and laser scattering into two components, which are detected separately, resulting in high cost and scattered device structure, which affects the test efficiency.

In addition, in the traditional laser scattering method, when the cells pass through the flow cell, the laser is irradiated on the cells, and the cell scattering signal is received at a small angle. Since the laser scattering signal is weak and easily interfered, it is difficult to obtain accurate laser scattering signals. Only through photoelectric Multiplier tubes and fluorescent stains to enhance scatter signal

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