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A set of microsatellite primers for red panda genes

A technology of microsatellites and red pandas, applied in the field of microsatellite primers, can solve problems such as paternity testing, which has not seen systematic research, and achieve good polymorphism, time and cost savings, and good applicability

Inactive Publication Date: 2020-10-09
CHENGDU RES BASE OF GIANT PANDA BREEDING
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  • Application Information

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Problems solved by technology

[0003] At present, the research on red pandas has accumulated a lot of results. The researches on red pandas by researchers at home and abroad mainly focus on the diet, behavior, subspecies differentiation, distribution and diseases of red pandas. Systematic research has not yet been seen

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  • A set of microsatellite primers for red panda genes
  • A set of microsatellite primers for red panda genes
  • A set of microsatellite primers for red panda genes

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Embodiment Construction

[0030] The present invention will be further described in detail below in conjunction with the examples, but the embodiments of the present invention are not limited thereto.

[0031] DNA was extracted according to the phenol-chloroform extraction method, and 25 pairs of microsatellite primers were used, as shown in the table below. The reaction conditions are: total system 10uL, DNA 50ng, 1 / 1.5U Taq polymerase 0.2uL), 20μM primer 0.2ul, 0.6 / 0.8mM Mg2+, 0.2mM dNTP MIX, 1.0uL 10×buffer. The reaction conditions were as follows: pre-denaturation at 95°C for 10 min; denaturation at 94°C for 15 s, Tm value 15 s, extension at 72°C for 30 s, a total of 10 cycles; denaturation at 89°C for 15 s, Tm value 15 s, extension at 72°C for 30 s, a total of 20 cycles, and finally 72°C. Extended at ℃ for 30min, stored at 4℃. Each reaction was repeated more than 3 times to ensure the consistency of the results.

[0032] Microsatellite Primer Information

[0033]

[0034]

[0035] In 2014...

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Abstract

The invention discloses a set of microsatellite primers of red panda gene. The 25 pairs of primers are designed aiming at repeated microsatellite sites from the third base to the sixth base. The phenomenon of slipping chain in primer amplification can be well avoided. The stability is high. The primers have good polymorphism. Through the 25 pairs of red panda microsatellite primers, red panda microsatellite sites with a high content of polymorphism are obtained, a genetic typing technology corresponding to the markers is established, and the primers can be applied to the paternity tests and individual identification of red panda and assist the management of captive populations of red panda.

Description

technical field [0001] The invention relates to a DNA molecular genetic marker and its application, in particular to a group of microsatellite primers for red panda genes. Background technique [0002] As a second-class national protected animal, the red panda is also listed in Appendix I of the Convention on International Trade in Endangered Species of Wild Fauna and Flora. Therefore, the protection of the red panda needs to be highly valued. At present, the red panda's habitat is fragmented, overhunting and hunting occur from time to time, and the habitat tends to degenerate towards the mountains. The research and protection of the red panda is of great significance to the study of the geological evolution of the earth and the evolution of animal systems. [0003] At present, the research on red pandas has accumulated a lot of results. The researches on red pandas by researchers at home and abroad mainly focus on the diet, behavior, subspecies differentiation, distributio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 张亮张志和沈富军侯蓉张文平杨智
Owner CHENGDU RES BASE OF GIANT PANDA BREEDING
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