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A microfluidic cell drug concentration gradient generator

A drug concentration, microfluidic technology, applied in biochemical instruments, enzymology/microbiology devices, detection of programmed cell death, etc., can solve problems such as poor scalability, complex structure, lengthy chip channels, etc. Concentration produces quicker, more efficient effects

Active Publication Date: 2019-04-23
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the number of concentrations = the number of layers + 2, if you want to get different concentrations, you will need a lot of layers, which will lead to lengthy chip channels and complex structures
This makes its scalability poor and it is difficult to achieve high-throughput biological analysis
In addition, the flow rate has a great influence on the stability of the concentration gradient (a good gradient can be formed when the flow rate is 0.001m / s-0.01m / s, and when the flow rate is greater than 0.01m / s, the gradient changes significantly), which is only suitable for ultra-low speed. cell analysis

Method used

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  • A microfluidic cell drug concentration gradient generator
  • A microfluidic cell drug concentration gradient generator
  • A microfluidic cell drug concentration gradient generator

Examples

Experimental program
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Embodiment Construction

[0018] Such as figure 1 , the embodiment of the present invention includes four groups of liquid inlet holes; each group of liquid inlet holes includes two liquid inlet holes communicated through the first microchannel 11; the first microchannel 11 communicates with the second microchannel 12; two of them The first microchannel 11 is vertically arranged, and after the two second microchannels 12 that are communicated with the two first microchannels are horizontally arranged and communicated, a common microchannel 13 is formed, and the two first microchannels are respectively connected with one Bending microchannel 14 is communicated; All the other first microchannels 11 are arranged horizontally, and the second microchannels communicated with these first microchannels 11 are all communicated with said common microchannel 14; The mixing channels 9 are in communication; all the micro-mixing channels 9 and the bending micro-channels 14 are in communication with one chamber 10 re...

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Abstract

The invention discloses a microfluidics cell drug concentration gradient generator. The microfluidics cell drug concentration gradient generator comprises four groups of liquid inlet holes which are communicated through microchannels so as to form drug and culture medium laminar flow combinations of different volume proportions in the microchannels; one group of microchannels with micro-mixing function can ensure that drug and culture medium laminar flow combinations of different volume proportions can be fully mixed to finally form different concentrations which can form nine concentration gradients together with liquid concentrations in two channels led out from a liquid inlet directly; one group of chambers are connected with the micro-mixed channels for cell culturing and drug interaction research, and nine chambers are independent from one another. The concentration gradient generator is hardly influenced by liquid inlet rate during the generation of concentration gradients, good linear concentration gradients can be formed, and the concentration gradient generator has a simple structure and provides a brandnew platform for screening and detection of cell drugs.

Description

technical field [0001] The invention relates to the field of designing microfluidic chips, in particular to a drug concentration gradient generator for cells. Background technique [0002] Drug screening, as the initial process and key step of new drug research, is to screen out high-efficiency new drugs or lead compounds from natural or synthetic compounds, and evaluate the biological activity, pharmacological effect and medicinal value of substances that may be used as drugs [1]. With the rapid development of cell biology technology, its microquantification and ultra-high-throughput advantages provide a way for high-throughput new drug screening based on the cell level [2]. High-throughput screening based on cell models can monitor and quantitatively analyze a variety of cellular events at the population cell level or single cell level[3] through a single experiment, providing reliable information for the initial screening of drugs. Traditional high-throughput screening ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/34
CPCG01N33/5008G01N2500/00
Inventor 刘正春石环环董波聂凯旋阳军亮龙梦秋徐慧
Owner CENT SOUTH UNIV
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