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Use method of drug screening biochip with air chamber

A biochip and air chamber technology, applied in drug screening, biochemical equipment and methods, compound screening, etc., can solve the problem of inability to observe cell morphological changes in real time, and achieve the effect of ensuring reliability.

Active Publication Date: 2017-05-31
CHONGQING UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, traditional drug screening chips cannot observe the morphological changes of cells under the action of drugs in real time.

Method used

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  • Use method of drug screening biochip with air chamber
  • Use method of drug screening biochip with air chamber
  • Use method of drug screening biochip with air chamber

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment 1

[0023] refer to figure 1 The chip shape structure, the fluid channel of the lower chip 20 has a height of 800 μm and a width of 300 μm. Each culture area 7 has a diameter of 5 mm, and a "U"-shaped structure 16 with a width of 60 μm semicircular arc wall in the middle, which is 5 μm away from the inner wall of the cylindrical culture area 7 .

[0024] The operation steps are as follows:

[0025] The first step, cleaning: first block the gas outlet 14, pass high-purity nitrogen gas into the gas inlet 3 for 1 hour, the gas passes through the gas chamber channel 4 to the gas chamber 9, and then passes through the gas channel 8 to two "V"-shaped injection ports One 1 and "V" type inlet two 17, and outlet 12 are discharged. Open gas outlet 14 subsequently, block two " V " type inlet one 1 and " V " type inlet two 17 , and outlet 12 , continue to feed high-purity nitrogen 1h, gas is discharged by gas outlet 14 . In order to achieve the purpose of removing impurity gas in the chip....

specific Embodiment 2

[0030] refer to Figure 1-Figure 3 The chip shape structure, the fluid channel of the lower chip 20 has a height of 800 μm and a width of 300 μm. Each culture area 7 has a diameter of 5 mm, and a "U"-shaped structure 16 with a width of 60 μm semicircular arc wall in the middle, which is 5 μm away from the inner wall of the cylindrical culture area 7 .

[0031] The operation is the same as that of the specific embodiment 1, the difference is that: the first step is to feed high-purity nitrogen for 1 hour in total, and continuously feed tertiary deionized water for 2 hours. The second step is to pass through the cell culture medium for 0.5h.

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Abstract

The invention relates to a use method of a drug screening biochip with an air chamber. The use method comprises the following steps: (1) cleaning; (2) carrying out experiment sample feeding; (3) carrying out drug administration and screening detection; and (4) cleaning after the experiment is finished. By operating and using the drug screening biochip with the air chamber, a good cell culture base is provided; and by virtue of structural improvement, a micro-fluidic technique and a cell culture technique are combined to be applied to the drug screening field, and the same cell growth environment is provided on one chip. According to the operation, the type of drugs or the drug administration amount is singly changed without changing other variables, and the drugs are screened by observing the change of physical signs, such as the cell state, under drug action.

Description

technical field [0001] The invention relates to a method for using a novel biological microanalysis chip for drug screening. In terms of use, it is a method for using a novel drug screening biochip with an air chamber, which is used in a living cell culture system. Background technique [0002] High-throughput drug screening refers to experimental methods based on molecular and cellular levels, using microplates as experimental tool carriers, using automated operating systems to perform experimental procedures, using sensitive and fast detection instruments to collect experimental data, and using computers to analyze The experimental data is analyzed and processed, tens of millions of samples are tested at the same time, and the technical system of the whole system is supported by the corresponding database. High-throughput drug screening system is an important field of new drug development research in innovative drug screening, and has been widely used in the screening of b...

Claims

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Application Information

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IPC IPC(8): C12M1/00
CPCB01L3/5027B01L2200/10C12N2503/02G01N33/5026G01N2500/10
Inventor 廖晓玲徐文峰罗贤解文月
Owner CHONGQING UNIVERSITY OF SCIENCE AND TECHNOLOGY
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