Method for recovering protein from heparin sodium extracted waste liquid

A technology of heparin sodium and protein, which is applied in the preparation methods of peptides, chemical instruments and methods, water/sludge/sewage treatment, etc., can solve the problems of waste of crude protein, immaturity, long cycle, etc., and reach the applicable pH value range. Wide, strong bridging adsorption performance, fast flocculation and sedimentation effect

Inactive Publication Date: 2017-05-31
QINGDAO JIULONG BIO PHARMA
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  • Application Information

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Problems solved by technology

The amino acid content in wastewater is high, and the chemical oxygen consumption is also relatively high, so it is not suitable for direct discharge
In addition, the casing-heparin processing wastewater also has a foul smell, which seriously affects people's living environment
At present, my country's resource utilization measures for heparin extraction wastewater are not mature enough, and the crude protein and organic matter in heparin sodium extraction wastewater cannot be fully utilized.
According to statistics, about 3 million tons of heparin extraction wastewater result in a waste of about 36,000 tons of crude protein every year
In recent years, there have been many heparin extraction wastewater treatment technologies in China, most of which use biological treatment processes, but the treatment cycle is long and the pollutants are not completely removed

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Extract 5000L of heparin sodium wastewater, temperature 48°C, pH 8.5, salt content 5.0%, add 30kg of aluminum trichloride solution (concentration 50%), stir for 10 minutes, after stirring evenly, add 20kg of polyacrylamide solution (concentration 10%), continue to stir for 10 minutes, let it stand for 30 minutes, the protein precipitates to the bottom, the supernatant is clarified, the supernatant solution is pumped off, the bottom protein is collected, dried, and weighed, the weight is 78kg. The COD removal rate was 96%, and the protein recovery rate was 98%.

Embodiment 2

[0021] Extract 8000L of heparin sodium wastewater, temperature 46°C, pH 8.0, salt content 4.4%, add 40kg of aluminum oxide solution (concentration 50%), stir for 10 minutes, add polyacrylamide solution 25kg (concentration 10% ), continue to stir for 10 minutes, let stand for 30 minutes, the protein precipitates to the bottom, the supernatant is clarified, the supernatant solution is sucked off, the bottom protein is collected, dried, and weighed, the weight is 102kg. The COD removal rate was 97%, and the protein recovery rate was 99%.

Embodiment 3

[0023] Add 4000L of waste water from heparin sodium extraction, temperature 46°C, pH 8.3, salt content 4.6%, add 20kg of aluminum trichloride solution (concentration: 50%), stir for 10 minutes, after stirring evenly, add 13kg of polyacrylamide solution (concentration 10%), continue to stir for 10 minutes, let it stand for 30 minutes, the protein precipitates to the bottom, the supernatant is clarified, the supernatant solution is pumped off, the bottom protein is collected, dried, and weighed, the weight is 50kg. The COD removal rate was 95%, and the protein recovery rate was 98%.

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PUM

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Abstract

The invention relates to a method for recovering protein from heparin sodium extracted waste liquid. The method comprises the steps that an aluminum trichloride solution is added to the heparin sodium extracted waste liquid; uniform stirring is performed; a flocculant solution is added; stirring is performed; standing is performed when substantive flocculent precipitates appear; a supernatant is removed; a precipitate is collected; and drying is performed to obtain the protein. High-molecular-weight polyacrylamide forms bridging between adsorbed particles and generates floccules, so that particle subsidence is facilitated; the dosage is small; the efficiency is high; and aftertreatment is easy.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to bioseparation, a method for extracting protein from heparin sodium waste liquid. Background technique [0002] Heparin sodium is a mucopolysaccharide widely present in mammalian tissues. Heparin sodium is the sodium salt of heparin. functions such as cancer. As a natural anticoagulant substance, heparin sodium has been valued by countries all over the world. It is also one of the main export drugs in my country. Crude heparin sodium is a traditional export product of our country and occupies an important position in the world. [0003] At present, heparin sodium is mainly extracted from the small intestinal mucosa of pigs. The wastewater after extraction of heparin sodium is rich in animal protein, fat and other organic substances that are difficult to degrade. The amino acid content in wastewater is high, and the chemical oxygen consumption is also relatively high, so it is not...

Claims

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Application Information

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IPC IPC(8): C07K1/30C02F1/52C02F1/56
CPCC07K1/30C02F1/5245C02F1/56C07K1/303
Inventor 刘乃山鹿凤岐迟培升
Owner QINGDAO JIULONG BIO PHARMA
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