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Method for extracting and purifying cytisine from Sophora tonkinensis

A technology of cytisine and pea, which is applied in the direction of organic chemistry, can solve the problems of low purity, high cost and long cycle of extraction and purification of cytisine, and achieve the effect of short cycle, simple operation and simple regeneration

Inactive Publication Date: 2017-05-31
南宁馨艺荣生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention utilizes advanced ultrasonic extraction technology to overcome the problems of low purity, high cost, heavy pollution and long cycle of extracting and purifying cytisine in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Weigh 50g of the mountain bean root medicinal material, crush it through a 60-mesh sieve, add 20 times the amount of 60% methanol solution, ultrasonically extract twice at 40°C for 40 minutes each time, filter, combine the filtrates, concentrate under reduced pressure to recover methanol, and use 5mol / Adjust the value to 4 with L hydrochloric acid, filter with suction, concentrate the filtrate to 0.1mg / mL, add it to a D101 macroporous resin column and let it stand for adsorption for 30 minutes; 50% ethanol, 70% ethanol elution of 5 times amount column volume, and elution speed is 1mL / min, collects 70% ethanol eluate, concentrates to dryness under reduced pressure, obtains the total alkaloid extract of Radix Solanum; The extract was dissolved in ethyl acetate-ethanol solution to a concentration of 0.1mg / mL, stirred into silica gel for complete adsorption, evaporated the solvent, added to a good silica gel column in a wet method, and used 5 times the column volume of acet...

Embodiment 2

[0022] Weigh 100g of the mountain bean root medicinal material, crush it through a 40-mesh sieve, add 15 times the amount of 80% ethanol solution, ultrasonically extract 3 times at 45°C for 30 minutes each time, filter, combine the filtrates, concentrate under reduced pressure to recover ethanol, and use 5mol / Adjust the value to 5 with L hydrochloric acid, filter with suction, concentrate the filtrate to 0.15mg / mL, add it to the LSI010 macroporous resin column and let it stand for adsorption for 40 minutes; 50% ethanol, 75% ethanol elution of 8 times the volume of the column, the elution rate is 1.5mL / min, collect the 75% ethanol eluate, concentrate to dryness under reduced pressure, and obtain the total alkaloid extract of Solanum root; Take the extract, dissolve it with ethyl acetate-ethanol solution to a concentration of 0.15 mg / mL, stir it into silica gel for complete adsorption, evaporate the solvent, add it to a good silica gel column in a wet method, and use 8 times the...

Embodiment 3

[0024] Weigh 200g of the mountain bean root medicinal material, crush it through a 20-mesh sieve, add 20 times the amount of 90% ethanol solution, ultrasonically extract 3 times at 50°C for 30 minutes each time, filter, combine the filtrates, concentrate under reduced pressure to recover ethanol, and use 5mol / Adjust the value to 6 with L hydrochloric acid, filter with suction, concentrate the filtrate to 0.2mg / mL, add it to the LSI010 macroporous resin column and let it stand for adsorption for 60 minutes; use 5 times the column volume of purified water, 5 times the column volume 50% ethanol, 80% ethanol elution of 10 times the amount of column volume, the elution speed is 2mL / min, collects 80% ethanol eluate, concentrates to dryness under reduced pressure, obtains the total alkaloid extract of the root of the mountain bean root; The extract is dissolved in ethyl acetate-ethanol solution to a concentration of 0.20 mg / mL, stirred into silica gel for complete adsorption, evapora...

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PUM

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Abstract

The invention discloses a method for extracting and purifying cytisine from Sophora tonkinensis. The method comprises the following steps: extraction: a Sophora tonkinensis medicinal material is pulverized, an aqueous solution of an organic solvent is used for extraction with ultrasound, the extract is filtered, filtrate is condensed, and a macroporous resin column is used for separating a Sophora tonkinensis total alkaloid extract product; separation: the alkaloid extract product is absorbed and separated by silica gel, and recrystallization is carried out in order to obtain the product. The method has the advantages of simple process, simple operation, and low cost; the materials used for separation are simple to regenerate without toxicity and pollution; the method is suitable for industrial large-scale production.

Description

technical field [0001] The invention relates to the field of traditional Chinese medicine chemistry, in particular to a method for extracting and purifying cytisine from the root of the mountain bean. Background technique [0002] Shandougen, also known as Guangdougen, is the dried root and rhizome of the leguminous plant Sophora tonkinensis Gagnep. It is a traditional Chinese medicine in my country. It has the effects of clearing away heat and detoxification, relieving throat and reducing swelling. It is used to treat accumulation of fire and poison, sore throat, swollen gums, damp-heat jaundice, arrhythmia, hepatitis, and liver cancer. Bean root is rich in alkaloids, flavonoids, triterpenoids and other active ingredients. [0003] Cytisine is an alkaloid component in mountain bean root, and its molecular formula is C 11 h 14 0N 2 , the molecular weight is 190.24. Cytisine has significant biological activity. It is clinically used for intravenous injection of 0.15% aqu...

Claims

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Application Information

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IPC IPC(8): C07D471/18
CPCC07D471/18
Inventor 邓刚梁可文黄能黄健军
Owner 南宁馨艺荣生物科技有限公司
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