Application of codonopsis pilosula pectic polysaccharides (CPP1c) and drug and health product thereof
A technology of pectin polysaccharides and health products, applied in the field of biomedical engineering, can solve the problems of low medicinal value and health care value, and achieve the effects of promoting the secretion of immune-related molecules, promoting the expression of immune-related genes, and promoting the expression of immune-related proteins
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Embodiment 1
[0068] This example provides the preparation and detection methods of Codonopsis pectin polysaccharide CPP1c.
[0069] The extraction method of Codonopsis pectin polysaccharide CPP1c comprises the following steps:
[0070] 1.1 Preparation and extraction of Codonopsis polysaccharide CPP1: Codonopsis pilosula is crushed, degreased with 95% ethanol, extracted in hot water at a temperature above 90°C, concentrated, and precipitated with ethanol with a final concentration of 10-40% , filtered, precipitated as crude Codonopsis polysaccharide CPP1, and protein and pigment were removed.
[0071] 1.2 Weigh 100 mg of the CPP1 sample after deproteinization and depigmentation, dissolve it in 100 mL of water filtered through a 0.45 μm filter membrane, centrifuge (3000 rpm, 3 min), and take the supernatant. After the DE-52 cellulose column was equilibrated with water filtered through a 0.45 μm filter membrane (about 12 h), the above prepared solution was loaded with a flow rate of 25 mL / h....
Embodiment 2
[0092] This example provides the application of Codonopsis pectin polysaccharide CPP1c in the preparation of drugs for promoting lymphocyte proliferation.
[0093] In vitro experiments
[0094] Part I, Preparation of Lymphocytes
[0095] Separate the spleen of SAMP8 mice or normal mice obtained under sterile conditions with lymphocyte separation medium, transfer it to a centrifuge tube, and carefully add 1640 medium along the tube wall to make it form with the separation medium interface. Centrifuge at 3000rpm for 0.5h. Carefully suck out the misty splenocyte layer with a pipette, wash with culture medium, and adjust the cell concentration to the desired concentration.
[0096] Part II, Lymphocyte Proliferation Experiment
[0097] 1.1 Experimental grouping, normal group: ordinary mouse spleen lymphocytes + blank medium; model group: SAMP8 mouse spleen lymphocytes + blank medium; Codonopsis pectin polysaccharide CPP1c administration group: SAMP8 mouse spleen lymphocytes + C...
Embodiment 3
[0105] This example provides the application of Codonopsis pectin polysaccharide CPP1c in the preparation of drugs for improving lymphocyte activity.
[0106] In vitro experiments
[0107] Refer to the mouse lymphocytes and grouping prepared in Example 2.
[0108] Carry out the experiment of in vitro lymphocyte activity; The method is as follows:
[0109] 1.1 Plant the splenic lymphocyte suspension of ordinary mice or SAMP8 mice in 24-well plates, 500 μL per well (about 1×10 7 indivual).
[0110] 1.2 According to the normal group: ordinary mouse spleen lymphocytes + blank medium; model group: SAMP8 mouse spleen lymphocytes + blank medium; Codonopsis pectin polysaccharide CPP1c administration group: SAMP8 mouse spleen lymphocytes + CPP1c (CPP1c The final concentration is 50 μg / mL, 100 μg / mL, 200 μg / mL) into groups; add blank culture medium to the normal group and model group respectively, and add gradient concentration of Codonopsis pectin polysaccharide CPP1c to the Codonop...
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