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Dalbergia hupeana tissue culture seedling rooting inducing method

A technology of Dalbergia group and Dalbergia, which is applied in the field of rooting induction of Dalbergia tissue culture seedlings, can solve the problems of not thick root system, small number of roots, uneven rooting, etc., and achieve shortening rooting cycle, fast rooting speed and many root systems Effect

Inactive Publication Date: 2017-05-31
唐春艳 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the process of tissue culture, the problems of low rooting rate, inconsistent rooting, small number of roots and not strong root system often appear.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Select Dalbergia subculture buds that have been cultured for 35-40 days in conventional tissue culture, and after disinfecting the surface of the bottle, select a single bud that grows robustly and has a height of 1-2 cm in the aseptic space on the ultra-clean workbench. , cut at 2-3 mm below the node, and remove the base leaves and petioles. The pruned single buds were inoculated in the pre-rooting medium, and the raw material content of the pre-rooting medium was: 1 / 2 improved ER medium+NAA 1.0mg / L+vitamin C 6g / L++VC 2.0mg / L + carrageenan 25g / L + agar 5.0g / L, carry out pre-rooting culture in an environment with a temperature of 20±1°C, a humidity of 55-60%, a light intensity of 2000lux, and a light of 16h / d. After the single bud has been pre-rooted for 30-35 days, the single bud is transferred to the rooting medium. The raw material content of the rooting medium is: 1 / 2 improved ER medium + IAA 1.0mg / L + ABT1#0.5 mg / L + sucrose 25g / L + agar 5.0g / L, rooting culture w...

Embodiment 2

[0025] Select Dalbergia subculture buds that have been cultured for 35-40 days in conventional tissue culture, and after disinfecting the surface of the bottle, select a single bud that grows robustly and has a height of 1-2 cm in the aseptic space on the ultra-clean workbench. , cut at 2-3 mm below the node, and remove the base leaves and petioles. The pruned single buds were inoculated in the pre-rooting medium, and the raw material content of the pre-rooting medium was: 1 / 2 improved ER medium+NAA 1.5mg / L+vitamin C 6g / L++VC 1.0mg / L + carrageenan 25g / L + agar 5.0g / L, carry out pre-rooting culture in an environment with a temperature of 20±1°C, a humidity of 60-65%, a light intensity of 2000lux, and a light of 15h / d. After the single bud has been pre-rooted for 30-35 days, the single bud is transferred to the rooting medium. The raw material content of the rooting medium is: 1 / 2 improved ER medium + IAA 1.5mg / L + ABT1#1.0 mg / L+sucrose 25g / L+agar 5.0g / L, rooting culture was c...

Embodiment 3

[0028] Select Dalbergia subculture buds that have been cultured for 35-40 days in conventional tissue culture, and after disinfecting the surface of the bottle, select a single bud that grows robustly and has a height of 1-2 cm in the aseptic space on the ultra-clean workbench. , cut at 2-3 mm below the node, and remove the base leaves and petioles. The pruned single buds were inoculated in the pre-rooting medium. The raw material content of the pre-rooting medium was: 1 / 2 improved ER medium+NAA 2.0mg / L+vitamin C 6g / L++VC 1.5mg / L + carrageenan 25g / L + agar 5.0g / L, carry out pre-rooting culture in an environment with a temperature of 20±1°C, a humidity of 65-70%, a light intensity of 2500lux, and a light of 15h / d. After the single bud has undergone pre-rooting culture for 30-35 days, the single bud is transferred into the rooting medium. The raw material content of the rooting medium is: 1 / 2 improved ER medium + IAA 2.0mg / L + ABT1#2.0 mg / L + sucrose 25g / L + agar 5.0g / L, rooti...

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Abstract

The invention discloses a dalbergia hupeana tissue culture seedling rooting inducing method which comprises the steps of choosing 35 to 40d dalbergia hupeana subculture buds cultured from general tissue culture strong buds, firstly performing pre-rooting culture after the strong buds are sterilized and pruned, then performing rooting culture after 30 to 35d pre-rooting culture, putting in an environment with temperature of 20+ / -1DEG C, humidity of 55 to70%, illumination intensity of 2000 to 2500lux and illumination of 15 to 16h / d to be cultured to obtain tissue culture seedlings with roots. The dalbergia hupeana tissue culture seedling rooting inducing method disclosed by the invention shortens a rooting period of the dalbergia hupeana subculture buds and has high rooting rate, multiple root systems and good quality; the rooting tissue culture seedlings have high transplanting survival rate; dalbergia hupeana tissue culture industrialized seedling culture is achieved, and excellent seedlings are provided for construction of artificial clone forest; the dalbergia hupeana tissue culture seedling rooting inducing method has better economical benefit, social benefit and ecological benefit.

Description

technical field [0001] The invention belongs to the asexual propagation technology of Dalbergia, and relates to a method for inducing rooting of Dalbergia tissue culture seedlings. Background technique [0002] Dalbergia hupeana Hance, Fabaceae, alias: I don't know spring, Wangshui sandalwood, sandalwood, etc., tree, 10-20 meters high, dark gray bark, peeling off in flakes, produced in Shandong, Jiangsu, Anhui, Zhejiang , Jiangxi, Fujian, Hubei, Hunan, Guangdong, Guangxi, Sichuan, Guizhou, and Yunnan, at an altitude of 600-1400 meters, can grow in plains and mountainous areas, likes light, is resistant to drought and barren, and does not choose soil, but it can be grown in deep, moist and well-drained areas The soil grows well, avoid saline-alkali soil; deep roots, strong germination, born in mountain forests or shrubs, common in mountain valleys and streams and slopes with small forests, its root bark, excavated in summer and autumn, tastes pungent and bitter, Xingping, sm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 唐春艳陈素云
Owner 唐春艳
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