Method for detecting proteins based on combination of colorimetric array sensor and mobile phone
An array sensor and protein technology, applied in the direction of color/spectral characteristic measurement, instruments, measuring devices, etc., can solve the problems of high cost, complicated operation, unfavorable site detection, etc., and achieve low cost, simple operation, and simplified signal processing Effect
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Embodiment 1
[0036] Example 1 The colorimetric array sensor used in connection with mobile phones is used for the detection of multiple proteins
[0037] Gold nanoparticles (AuNPs) are prepared by sodium citrate reduction of chloroauric acid. As nanometer materials with a particle size range of nanometer level, their synthesis is very simple. The classical method of AuNPs synthesized by citrate reduction of chloroauric acid is the most Convenient.
[0038] The method for detecting protein based on the combination of a colorimetric array sensor and a mobile phone provided in this embodiment includes the following steps:
[0039] 1. Preparation of gold nanoparticles: prepared by reducing chloroauric acid with sodium citrate, the molar ratio of chloroauric acid and sodium citrate is 3.5:1, and the average particle size of the gold nanoparticles in the resulting solution is 13nm.
[0040] 2. Preparation of protein-nano-gold complexes: add 50 μL of 0.5 μM 12 kinds of proteins to 100 μL of the above-men...
Embodiment 2
[0051] Example 2 Practical application of protein detection
[0052] In order to prove the feasibility of the array sensor in Example 1, in the urine of healthy humans diluted 1 times, 12 kinds of proteins at 200nM were detected. Since human urine contains about 155mM NaCl, the urine was diluted 6 Times, 3 times, 2 times and 1.5 times to achieve the NaCl concentration required by the sensor unit. To 200μL of AuNPs were added 100μL of healthy human urine diluted 6 times, 3 times, 2 times, and 1.5 times containing 1μM protein to be tested, and then added 200μL ultrapure water, incubated for 30min, UV detection, the results showed that it can be completely distinguish( Figure 5 ). Using the array sensor in the actual sample, the color difference map corresponding to 200nM multiple proteins is shown in the mobile phone photograph Image 6 .
[0053] Subsequently, the quantification of HSA and Lys and the detection of their mixture were carried out respectively. The photos taken by ...
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