A kind of method for improving nikkomycin production
A technology of nikkomycin and inoculum volume, which is applied in the field of microorganisms and can solve the problems of large consumption of raw materials and low yield
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Embodiment 1
[0029] The invention provides a method for preparing nikkomycin by fermenting Streptomyces chromogenes BJX005, comprising the following steps:
[0030] Step (1): Prepare the seed liquid of Streptomyces chromogenes, and inoculate the seed liquid in a fermentation medium with an inoculation amount of 4% to carry out fermentation culture to obtain a fermentation liquid; the medium contains the following: Components: 3g corn starch, 3g soybean meal powder, 0.2g yeast powder, 0.6g sodium chloride, 0.04 ammonium sulfate, 0.5g calcium carbonate, polysiloxane defoamer SXP-101-10.02g, Tween 800.02g , add water to 100g. Heat to 80°C to dissolve, and after cooling, use sodium hydroxide to adjust the pH value to 6.8; the fermentation culture is shaking culture, and the rotation speed of shaking culture is 220r / min, the fermentation time is 70h, and the fermentation temperature is 28°C; the fermentation vessel uses 8 After sealing with a layer of gauze, sterilize at 121°C for 30 minutes; ...
Embodiment 2
[0034] The invention provides a method for preparing nikkomycin by fermenting Streptomyces chromogenes, comprising the following steps:
[0035] Step (1): Prepare the seed liquid of Streptomyces chromogenes, and inoculate the seed liquid in a fermentation medium with an inoculum of 10% to carry out fermentation culture to obtain a fermentation liquid; the medium contains the following Components: cornstarch 2.5g, soybean meal powder 2g, yeast powder 0.2g, sodium chloride 0.4g, ammonium sulfate 0.05, calcium carbonate 0.45g, polysiloxane defoamer DF-805 (purchased from Guangdong Dongguan De Feng defoamer factory) 0.025g, Tween 800.01g, add water to 100g. Heat to 80°C to dissolve, and after cooling, use sodium hydroxide to adjust the pH value to 7.5; the fermentation culture is shaking culture, and the rotation speed of shaking culture is 200r / min, the fermentation time is 80h, and the fermentation temperature is 25°C; the fermentation vessel is 10 After sealing with a layer of...
Embodiment 3
[0039] Compared with Example 1, the only difference is that the defoamer used in this example is a GPE polyether defoamer, i.e. foam enemy, and other conditions are the same.
[0040] The nikkomycin prepared in this example was tested, and the yield was 40300 μg / mL.
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