The pgag gene regulating pomegranate flower ovule development and its use

A technology of pomegranate flower and gene, applied in the field of PgAG gene

Inactive Publication Date: 2019-12-10
ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, so far no information on the isolation of pomegranate PgAG Related reports on the influence of genes on flower development

Method used

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  • The pgag gene regulating pomegranate flower ovule development and its use
  • The pgag gene regulating pomegranate flower ovule development and its use
  • The pgag gene regulating pomegranate flower ovule development and its use

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Embodiment Construction

[0021] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

[0022] of the present invention PgAG The gene is cloned and extracted from Tunisian soft seed pomegranate, its nucleotide sequence is as follows: SED IDNO: 1 (sequence 1), and its amino acid sequence is as SED IDNO: 2 (sequence 2).

[0023] 1. Total RNA extraction and first-strand cDNA synthesis

[0024] The total RNA was extracted using the improved CTAB method, and the RNA concentration and OD260 / 280 value were measured with a Thermo Scientific Nanodrop 1000 mic...

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Abstract

The invention relates to a PgAG gene cloned from a pomegranate and used for regulating and controlling pomegranate blossom ovule development and application thereof. A nucleotide sequence of the gene is shown as SED ID NO:1, and an amino acid sequence of the gene is shown as SED ID NO:2. After the PgAG gene is in forward-direction connection with a carrier pBI121, a PBI121-PgAG recombinant expression vector is obtained, the PBI121-PgAG recombinant expression vector is converted into an agrobacterium stain GV3101, the ground part of an arabidopsis thaliana plant is inversely arranged in a dip dyeing solution, dark culture is performed overnight, hen normal cultivation management can be performed, dip dyeing can be performed one time after one week for waiting for harvesting T0-generation transgenic seeds, T1-generation plants are presented to have smaller petals, shorter stamens, thick and strong pistil and longer style head surface papillary cells.

Description

technical field [0001] The present invention relates to a kind of pomegranate flower ovule regulation and control method cloned from pomegranate PgAG Genes and their uses. Background technique [0002] Pomegranate( Punica granatum L.) Originated in Central Asia, it is widely cultivated all over the world and has high processing and health value. Studies have shown that pomegranate and its processed products have important preventive and therapeutic effects on cancer, atherosclerosis, coronary heart disease, hypertension, hyperlipidemia, AIDS, and infectious diseases. Severe pistil abortion occurs during the development of pomegranate flower, and pistil abortion is the key factor restricting the fruit yield of pomegranate flower. There are two main ways to control the development of pistil in plants: the first is WUS-AG Related genes jointly regulate pistil development; the second is KNOXI Gene-like regulation of pistil development. The first pathway is the main reg...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/84A01H5/02A01H6/20
CPCC07K14/415C12N15/827
Inventor 陈利娜曹尚银李好先刘贝贝牛娟
Owner ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
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