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Novel-type tribonema and cultivation and application thereof

A technology of yellow silk algae and a cultivation device, which is applied in the application, seaweed cultivation, cultivation and other directions, can solve the problems such as difficulty in further improving the yield, difficulty in realizing industrial independence, and restricting industrial application, etc., and achieves high oil yield. , easy to harvest, avoid the effect of flocculation additives

Active Publication Date: 2017-05-24
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the algae strains used in this patent are common Xanthophyllum algae strains or those purchased from foreign algae bank. Due to the limitation of strains, it is difficult to further increase the yield; and the algae strains purchased from foreign algae species banks need to be authorized for commercial application in the future, it is difficult to achieve industrial autonomy, and the economic cost is extremely high, which limits its industrial application

Method used

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  • Novel-type tribonema and cultivation and application thereof
  • Novel-type tribonema and cultivation and application thereof
  • Novel-type tribonema and cultivation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1: Isolation, purification and identification of algae strain Tribonema sp.PD2

[0043] 1 material

[0044] 1.1 Separation of sources

[0045] River water in the suburbs of Pingdu City, Qingdao.

[0046] 1.2 Medium

[0047] BG-11 medium: NaNO 3 1.5g / L, MgSO 4 ·7H 2 O 75mg / L, CaCl 2 2H 2 O 36mg / L, citric acid 6mg / L, Na 2 EDTA 1mg / L, ferric ammonium citrate 6mg / L, Na 2 CO 3 20mg / L, K 2 HPO 4 ·3H 2 O 47.5mg / L, H 3 BO 3 2.86mg / L, ZnSO 4 ·7H 2 O 0.222mg / L, CuSO 4 ·7H 2 O 0.079mg / L, MnCl 2 4H 2 O 1.81mg / L, Na 2 MoO 4 0.39mg / L, Co(NO 3 ) 4 ·6H 2 O 0.0494mg / L.

[0048] 2 methods

[0049] In the spring, 20 ml of surface water samples were taken from the river water in the suburbs of Pingdu City, Qingdao. Add 180ml of BG-11 medium to the water sample, place it in a glass bubble column and culture it for 5 days, the temperature is 20°C, and the light intensity is 100μmol photons m -2 the s -1 . The culture was filtered through a double laye...

Embodiment 2

[0051] Embodiment 2: the glass bubble column culture of algal strain Tribonema sp.PD2

[0052] Under laboratory conditions, inoculate the yellow hair algae Tribonema sp.PD2 cultured with BG-11 medium in advance into a glass bubble column with a diameter of 5cm and a volume of 800ml for cultivation, and the inoculated biomass is 0.1-0.2g / L , the ventilation volume is 0.05~0.5vvm (the carbon dioxide content is 1.5%), the concentration of sodium nitrate is 0~3g / L, the concentration of dipotassium hydrogen phosphate is 0~0.095g / L, the temperature is 20℃, and the light intensity is 100μmol photons m -2 the s -1 . The cultivation time was 18 days, during which, the biomass was measured every 3 days, the oil content was measured every 6 days, and the contents of sodium nitrate and dipotassium hydrogen phosphate in the algae liquid were measured every 3 days. The fatty acid composition of the algae harvested on the 18th day was analyzed after the oil was extracted.

[0053] Determi...

Embodiment 3

[0056] Embodiment 3: the outdoor open pond cultivation of algal strain Tribonema sp.PD2

[0057] Under outdoor conditions, inoculate the algae liquid of Tribonema sp.PD2 pre-cultured with BG-11 medium in an open pool of 6m×4m×0.2m, the depth of the algae liquid is 10-15cm, and the initial inoculated biomass is 0.1~0.2g / L, the ventilation rate is 0.05~0.5vvm (containing 5% boiler flue gas), the concentration of sodium nitrate is 1.5g / L, the concentration of dipotassium hydrogen phosphate is 0.047g / L, and the temperature is 15~ 35°C, the daytime light intensity is 50-1000μmol photons m -2 the s -1 . The culture time was 20 days, during which, the biomass was measured every 2 days.

[0058] Determination of biomass: Take a certain volume of algae liquid, filter it with a 0.45 μm filter membrane that has been weighed in advance, and rinse it with distilled water for several times, then place the filter membrane in an oven at 105°C to dry to constant weight, and weigh it. Deter...

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Abstract

The invention discloses a Tribonema sp. PD2 strain with a preservation number of CGMCC No.10488, and application thereof in the production of biodiesel, palmitoleic acid, feed, food, skin care products, and therapeutic drugs for cardiovascular and cerebrovascular diseases, and in the carbon dioxide emission reduction, and waste gas treatment and / or wastewater treatment. The Tribonema sp. PD2 strain has the advantages of fast growth, high biomass and easiness in harvesting, and is suitable for industrial application. The oil content of the Tribonema sp. PD2 strain is as much as 60% or more of the dry biomass, wherein the content of C16-C18 is as much as 85% of the total fatty acids, the content of the palmitoleic acid (C16:1omega7) is no less than 45% of the total fatty acids, and the content of the total unsaturated fatty acids is as much as 65% of the total fatty acids. Therefore the Tribonema sp. PD2 strain is especially suitable for the production of biodiesel and the development of health care products and foods.

Description

technical field [0001] The present invention relates to a new strain of Tribonema sp. PD2 and its cultivation and application. Specifically, the present invention relates to a strain of Tribonema sp.PD2 with high oil content, and using said strain for large-scale cultivation and for applications in the production of biofuels, feed, food, skin care, CO2 reduction, waste gas treatment and / or wastewater treatment. Background technique [0002] With the sharp decline of fossil energy reserves and the increasingly prominent environmental problems, the development of renewable clean energy has multiple meanings for alleviating the energy crisis and environmental degradation. Compared with other renewable energy sources, bioenergy has the characteristics of abundant sources, easy storage and transportation, and less secondary pollution. However, traditional bioenergy based on corn, soybean, sugarcane, etc. has been controversial due to food security issues, and it has become an ur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G33/00A61K8/98A61K8/97A61Q19/00
CPCY02A40/80Y02P60/20
Inventor 周文俊刘天中陈林汪辉王俊峰高莉丽张维程文涛
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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