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Thunia alba tissue culture seedling culture method

A technology of tissue culture and rock shoots, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of difficult restoration of wild resources, small and large seeds, and damage to the natural environment, and solve the problems of difficult reproduction and reproductive coefficient High, fast breeding effect

Active Publication Date: 2017-05-17
江苏知聚知识产权服务有限公司
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  • Application Information

AI Technical Summary

Problems solved by technology

Because the seeds of rock bamboo shoots have small and large seeds and no endosperm, they need suitable mycorrhizal fungi to germinate under natural conditions. The destruction of the natural environment and artificial over-excavation, coupled with harsh conditions for seed germination, have resulted in the recovery of wild resources in the original habitat. extremely difficult
[0003] At present, there is no method for raising seedlings by tissue culture of rock bamboo shoots, and there is no artificial planting and artificial seedling cultivation, which restricts the large-scale seedling cultivation and planting of rock bamboo shoots to a certain extent.

Method used

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Embodiment Construction

[0015] The invention is further described below in conjunction with specific embodiments:

[0016] A method for raising seedlings by tissue culture of rock bamboo shoots, comprising the following steps:

[0017] (1) Medium preparation: The medium includes germination induction medium, proliferation medium and rooting medium; among them, the germination induction medium is MS+NAA0.2mg / L+KT0.5mg / L+Huabao No.1 0.7g / L L+banana mash 30g / L+mashed potato 20g / L+sucrose 30g / L+agar 5g / L+PH5.9; proliferation medium is MS+IBA0.5mg / L+6-BA0.5mg / L+sucrose 30g / L+agar 5g / L+PH5.9; rooting medium is 2 / 3MS+NAA0.8mg / L+IAA0.5mg / L+sucrose 30g / L+activated carbon 0.5g / L+agar 5g / L+ph5.9; 60ml per bottle of culture medium and 120ml of rooting medium per bottle are packed into culture bottles, sterilized at 121°C and 0.5Mpa for 25min, cooled and solidified for later use.

[0018] (2) Preparation of explants: in autumn, pick plump fruits from mature plants as explants, and clean and disinfect them. The...

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Abstract

The invention discloses a thunia alba tissue culture seedling culture method, which comprises the following steps of (1) culture medium preparation; (2) explants preparation; (3) inoculation culture; (4) domestication. By using the method provided by the invention for thunia alba tissue culture seedling culture, the germination rate reaches 90 percent or higher; the breeding coefficient reaches 3 to 5; the characteristics of high breeding coefficient and high breeding speed are realized; in addition, the bred seedlings have the plant features identical to the parent body; the resistance is high. The problem of difficult breeding of the thunia alba is greatly solved. The scaled and factory production of the thunia alba are facilitated.

Description

technical field [0001] The invention relates to the technical field of growing seedlings by plant tissue culture, in particular to a method for growing seedlings by tissue culture of rock bamboo shoots. Background technique [0002] Rock bamboo shoots are the whole herb of Thunia alba (Lindl.) Rchb.f., a plant in the family Orchidaceae, also known as Tonglan, Yanjiao, Dianthus, and Yanzhu. Perennial epiphytic herb, about 30-50 cm high, with many fibrous roots, fleshy, off-white or light brown. Rock bamboo shoots are sweet in taste and flat in nature. According to "Yunnan Chinese Herbal Medicine", "Guizhou Medicine Plant Catalogue" and "Yunnan Simao Chinese Herbal Medicine Selection", it has the functions of relieving cough and asthma, promoting blood circulation and removing blood stasis, and is used to treat pneumonia, trachea and bronchitis , gastric and duodenal ulcers, fractures and bruises, etc. Rock bamboo shoots have high medicinal value and have a huge market prosp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 曹强周明辉任广标蔡凤相刘芳
Owner 江苏知聚知识产权服务有限公司
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