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Molecular genetic marker related with egg laying performance of hens and application

A molecular genetic marker, egg-laying performance technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc. High degree of precision, accelerated breeding process, simple operation effect

Pending Publication Date: 2017-05-10
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Abstract
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Problems solved by technology

Since phenotypic selection of eggs is a long-term accumulation process, there are many influencing factors, such as selection intensity, number of selected traits, generation interval, environmental conditions and nutritional levels, etc., all of which will have an effect on phenotypic selection, and its variation is continuous. In addition, the relationship between the phenotype of a trait and its genotype is complicated, and in most cases it is not completely consistent, so the selection is often difficult to be accurate. Only by repeating the same method many times, the directional selection for several generations can achieve significant results. There is a cycle Shortcomings such as length, low efficiency, high cost, and slow progress

Method used

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  • Molecular genetic marker related with egg laying performance of hens and application
  • Molecular genetic marker related with egg laying performance of hens and application
  • Molecular genetic marker related with egg laying performance of hens and application

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Embodiment

[0021] The method of utilizing molecular markers in the present invention to assist selection of chicken laying performance is obtained after completing the following basic work:

[0022] 1. Primers and probes

[0023] The primers and probes for FSH-β gene polymorphism detection are shown in Table 1 and Table 2:

[0024] Table 1 Primers for FSH-β gene polymorphism detection

[0025]

[0026] Table 2 FSH-β gene polymorphism primer probe

[0027]

[0028] 2. Study on SNP polymorphism by ligase detection reaction

[0029] Add TE to dilute to 50 pmol / μL according to the molecular weight of the primer and probe as the mother solution. For multiplex PCR and LDR, each primer mother solution is mixed in equal proportions to form a mixture solution that can be directly reacted. PCR amplifies the fragment where the SNP site is located, and prepare PCR Master Mix (20 μL system) according to the table below.

[0030] Table 3 PCR reaction system

[0031]

[0032] Add 200 μL ...

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Abstract

The invention provides a molecular genetic marker related with an egg laying performance of hens and application. An FSH (Follicle-Stimulating Hormone)-beta gene is used as the molecular genetic marker related with the egg laying performance of the hens; the molecular genetic marker is characterized in that the nucleotide sequence of the molecular genetic marker is shown as SEQ ID No. 1 or the sequence at least comprises a specific fragment of a 259 T>G mutated basic group. The egg laying performance of the hens are selected according to gene types and the molecular genetic marker has the characteristics of high accuracy, simplicity in operation and low cost (the selection cost is about 0.3 yuan per hen) and the like; automatic detection can be carried out; secondly, a molecular marking method provided by the invention can be used for selecting properties of the egg laying performance of the hens at an early life stage (within one week after chickens are hatched), the generation interval can be shortened, the selection intensity is improved and high-quality breeding hen parents can be selected relatively early, so that a breeding progress of the hens is accelerated; compared with a conventional determination selection method for the egg laying performance of the hens, the selection time of each generation can be shortened by 16 months.

Description

technical field [0001] The invention belongs to the technical field of molecular genetic marker-assisted selection of laying hens, and in particular relates to a molecular genetic marker for laying performance of chickens and an application thereof. Background technique [0002] my country's local broiler breeds are rich in resources, but compared with foreign commercial breeds, the overall egg production performance is poor. Improving the egg production performance of local broiler breeds has always been one of the main goals of poultry breeding in my country. The traditional breeding method for egg production performance of chickens is to select by measuring the egg production phenotype of a generation (500 days old). Since phenotypic selection of eggs is a long-term accumulation process, there are many influencing factors, such as selection intensity, number of selected traits, generation interval, environmental conditions and nutritional levels, etc., all of which will h...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 李国勤卢立志田勇陈黎沈军达陶争荣曾涛徐坚
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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