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Thermoanaerobacterium thermosaccharolyticum and application thereof to biological hydrogen production

A technology of saccharoanaerobic bacteria and biological hydrogen production, which is applied in the field of thermophilic saccharolytic anaerobic bacteria and its application in biological hydrogen production, can solve the problems of acid pretreatment liquid environmental pollution and high hydrogen production efficiency, and achieve the goal of producing Excellent hydrogen efficiency, broad application and promotion prospects, and the effect of efficient hydrogen production

Active Publication Date: 2017-05-10
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The strain can directly utilize xylose in the bagasse acid pretreatment solution, and has high hydrogen production efficiency, and this process does not require an additional acid pretreatment solution detoxification process, which greatly saves production costs and can solve the problem of acid pretreatment solution pollution to the environment

Method used

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  • Thermoanaerobacterium thermosaccharolyticum and application thereof to biological hydrogen production
  • Thermoanaerobacterium thermosaccharolyticum and application thereof to biological hydrogen production
  • Thermoanaerobacterium thermosaccharolyticum and application thereof to biological hydrogen production

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1: Isolation and identification of thermophilic saccharolytic anaerobic bacteria:

[0057] (1) Acquisition of unknown bacterium stable culture medium: the papermaking sludge (from a paper mill in Guangdong Province) that will be taken is made into 10% (w / v) with sterile 50mM phosphate buffer in the ultra-clean workbench The suspension was inoculated in the xylose liquid medium whose carbon source was 5g / L xylose at a ratio of 10% (v / v), placed in a shaker at 55°C for 120h, and then cultured with 10% (v / v) The proportion of v) was inoculated in the xylose liquid medium whose carbon source was 5g / L xylose in fresh carbon source, continued to cultivate for 72h, and repeated transfer culture 10 times, in order to obtain stable flora distribution, wherein, the xylose liquid medium The main ingredients are: xylose 5g / L (carbon source), ammonium chloride 1g / L, sodium chloride 1g / L, dipotassium hydrogen phosphate 1g / L, potassium dihydrogen phosphate 1g / L, cysteine ​​0.5...

Embodiment 2

[0070]Example 2: Determination of the ability of thermophilic saccharolytica anaerobic bacteria to produce hydrogen with different carbon sources

[0071] (1) Preparation of thermophilic anaerobic saccharolyticum seed solution: the culture solution of the isolated single colony obtained in step (4) of Example 1 was first passed through a 10mL cillin bottle (4.5mL seed medium was housed) at 55 ℃, 150rpm shaking culture for 18h to activate, and then in a 100ml serum bottle (with 45mL seed medium) at 55°C, 150rpm shaking culture for 18h for amplification to obtain seed liquid, wherein the seed medium is 5g / L xylose Culture medium (with the xylose medium in embodiment 1).

[0072] (2) Preparation and sterilization of fermentation media with different carbon sources: the fermentation media uses three carbon sources, namely 5g / L glucose, 5g / L cellobiose and 5g / L xylose, and other components except carbon sources The same as the above seed medium, put 27ml of fermentation medium int...

Embodiment 3

[0074] Example 3: Determination of Hydrogen Production Ability of Thermosaccharolyticum Anaerobic Bacteria with Different Concentrations of Xylose

[0075] (1) The preparation of the seed solution is the same as in Example 2.

[0076] (2) The preparation and sterilization of the fermentation medium were the same as in Example 2, except that the carbon sources of the fermentation medium were 2.5 g / L, 5 g / L, 7.5 g / L and 10 g / L of xylose respectively.

[0077] (3) The fermentative hydrogen production by thermophilic saccharolytic anaerobic bacteria is the same as in Example 2, and the results are as follows Figure 5 As shown, the yields of hydrogen after the fermentation are: 39.64mM, 71.57mM, 71.25mM, 60.38mM respectively, and the molar hydrogen yields converted into substrates are: 2.38mol, 2.15mol, 1.43mol and 0.91mol respectively, indicating that the separation The hydrogen production efficiency of the thermophilic saccharolytica anaerobic bacteria decreased with the increa...

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Abstract

The invention discloses thermoanaerobacterium thermosaccharolyticum and application thereof to biological hydrogen production. The name of the thermoanaerobacterium thermosaccharolyticum is thermoanaerobacterium thermosaccharolyticum MJI, and the thermoanaerobacterium thermosaccharolyticum with the preservation number of CGMCC No:60096 is preserved at the Microbial Strain Collection Center of Guangdong, Guangdong Institute of Microbiology on Floor 5, No. 59, No. 100 Courtyard, Xianlie Middle Road, Guangzhou City, Guangdong Province, the People's Republic of China on Oct. 31, 2016. The thermoanaerobacterium thermosaccharolyticum has the following advantages that pentose and hexose can be directly utilized, xylose in acid pretreatment liquid can be directly utilized, and an additional acid pretreatment liquid detoxification process is not required; the hydrogen yield is higher; the hydrogen production efficiency is higher. Therefore, the thermoanaerobacterium thermosaccharolyticum has wide application and popularization prospect in the biological hydrogen production.

Description

technical field [0001] The invention belongs to the technical field of hydrogen production by microbial fermentation, and in particular relates to a thermophilic saccharolytic anaerobic bacterium and its application in biological hydrogen production. Background technique [0002] With the progress of human society, the demand for energy is increasing day by day. At present, most of the world's energy supply is fossil energy, but due to its non-renewable, limited reserves and environmental pollution, it restricts the progress of human society. The energy and environmental crisis has pushed biomass energy to a research boom, hoping that it can replace fossil energy and support the energy demand of human society. Biomass energy uses renewable lignocellulose as raw material, and uses microorganisms or other means to convert it into renewable energy that can be directly used. Due to its renewable and environmental friendliness, people have high expectations and have attracted th...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P3/00C12R1/01
CPCC12P3/00C12P2203/00C12N1/205C12R2001/01
Inventor 朱明军胡彬彬
Owner SOUTH CHINA UNIV OF TECH
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